Figure 3
From: Proteome alteration induced by hTERT transfection of human fibroblast cells
Experimental design. Figure 3 shows an example of the experimental design applied for differential 2D-DIGE analysis and mass spectrometry based identification. Nuclear sub-proteomes from WI38, hTERT WI38 and HPV-WI38. Each spot is selected from the standardized abundance, then excised and digested for MS/MS analysis.