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Table 1 Identification of proteins expressed at equivalent levels in developing yeast and mould phases of P. marneffei.a

From: Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei

Spot (Fig.)

Protein Name

NCBI Accession (Version)

Theo. pI/M r (kDa)

Expt. pI/M r (kDa)

Species

Mascot Score

NP/PD

MS/MS Peptide Sequence

SC (%)

B27 (2, 3)

hypothetical protein (malate dehydrogenase)

XP_001817504 (gi|169766066)

8.79/35.7

5.8/52.6

Aspergillus oryzae

2597

9/122

R.VSELALYDIR.G

K.RLFGVTTLDVVR.A

R.LFGVTTLDVVR.A

R.MAESLLK.A + OM

R.IQFGGDEVVK.A

R.DDLFNTNASIVR.D

K.DGAGSATLSMAMAGAR.M + 2 OM

K.AKDGAGSATLSMAMAGAR.F + 2 OM

K.VELGPNGVEKILPVGQVNAYEEK.L

27

B29 (2, 3)

Cu,Zn-superoxide dismutase

ABD67502 (gi|89329757)

5.74/16.1

5.9/35.0

Penicillium marneffei

5183

9/139

M.VKAVAVLR.G

K.GTVTFEQADENSPTTISWNITGHDANAER.G

K.THGAPTDDER.H

K.THGAPTDDERHVGDLGNFK.T

R.HVGDLGNFK.T

K.TDAQGNAVGFVEDK.L

K.LIGAESVLGR.T

R.TIVVHAGTDDLGR.G

K.TGNAGPRPACGVIGISA.-

71

B30 (2, 3)

hypothetical protein (peroxiredoxin)

XP_001226164 (gi|116200704)

5.57/25.1

6.1/45.9

Chaetomium globosum

202

2/25

R.VVKPYLR.F

R.TILSYPASTGR.N

K.IAYLYDMIDYQDTTNVDEK.G + OM

R.KIAYLYDMIDYQDTTNVDEK.G + OM

16

B31 (2, 3)

hypothetical protein (nucleotide diphosphate kinase)

XP_001393898 (gi|145242650)

8.45/17.2

6.5/33.9

Aspergillus niger

413

5/127

R.GLVGPIISR.F

R.TILGATNPLASAPGTIR.G

R.GDYAIDVGR.N

R.NVCHGSDSVENAQK.E

K.HSQFDWIYEK.A

38

B33 (2, 3)

hypothetical protein (malate dehydrogenase)

XP_001246025 (gi|119190837)

9.19/36.0

6.1/45.9

Coccidioides immitis

328

5/9

R.FAESLLK.A

R.IQFGGDEVVK.A

R.VSQLALYDIR.G

R.LFGVTTLDVVR.A

R.DDLFNTNASIVR.D

14

B37 (-)

ATP synthase F1, beta subunit

XP_753589 (gi|70997705)

5.30/55.6

4.5/32.5

Aspergillus fumigatus

219

3/3

K.AHGGYSVFTGVGER.T

R.EGNDLYHEMQETGVIQLEGESK.V + OM

K.SLQDIIAILGMDELSEADKLTVER.A + OM

11

C27 (2, 3)

hypothetical protein (ketol-acid reducto-isomerase)

XP_363882 (gi|39952331)

8.22/44.9

6.3/54.9

Magnaporthe grisea

154

2/4

R.DNGLNVIIGVR.K

K.DDTLALIGYGSQGHGQGLNLR.D

8

C34 (2, 3)

peptidyl-prolyl cis-trans isomerase B precursor (cyclophilin)

XP_001728304 (gi|1164426123)

8.61/22.5

6.1/40.0

Neurospora crassa

69

3/4

R.IVMGLYGK.T + OM

K.GLLSMANAGK.D + OM

K.HVVFGEVLEGYDVVEK.I

16

D1 (2)

ATP synthase F1, subunit beta

XP_753589 (gi|70997705)

5.30/55.6

5.3/58.9

Aspergillus fumigatus

8540

22/277

K.LVDLK.D

K.IGLFGGAGVGK.T

K.LVDLKDTIR.S

K.VVDLLAPYAR.G

R.VQQMLQEYK.S + OM

K.IHQVIGAVVDVK.F

R.TIAMDGTEGLTR.G + OM

R.IVNVTGDPIDER.G

R.FTQAGSEVSALLGR.I

K.AHGGYSVFTGVGER.T

R.VALTGLTIAEYFR.D

K.TVFIQELINNIAK.A

K.VALVFGQMNEPPGAR.A + OM

R.GISELGIYPAVDPLDSK.S

R.DEEGQDVLLFIDNIFR.F

R.FLSQPFTVAQVFTGIEGK.L

K.SLQDIIAILGMDELSEADK.L

R.EGNDLYHEMQETGVIQLEGESK.V + OM

K.SLQDIIAILGMDELSEADKLTVER.A + OM

K.AIINGEGDDLPEAAFYMVGDFESAR.A + OM

R.TREGNDLYHEMQETGVIQLEGESK.V + OM

K.GSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSR.G

58

D11 (-)

3-isopropyl-malate dehydrogenase

XP_752944 (gi|70996378)

5.32/39.3

5.5/47.0

Aspergillus fumigatus

323

5/13

R.NVIEAGIR.T

R.GVDFNIIR.E

R.ELTGGIYFGDR.K

K.NADAVLLGAIGGPK.W

K.VNGIYEPIHGSAPDIAGK.G

16

E1 (2, 3)

RACK1-like protein

ABA33785 (gi|75993570)

6.59/35.5

6.3/51.3

Para-coccidioides brasiliensis

271

7/13

K.FTITDK.G

R.LWELATGNTTR.T

K.VDELKPEYVEK.G

K.DGTTMLWDLNESK.H + OM

K.SKVDELKPEYVEK.G

R.FSPNPQNPVIVSAGWDK.L

R.IQTDHIGHTGYINTVTISPDGSLCASGGK.D

28

E11 (2, 3)

hypothetical protein (aconitase)

XP_001393157 (gi|145241021)

6.18/84.9

5.7/66.2

Aspergillus niger

536

10/21

K.DIILK.V

K.FNPLTDK.L

K.GNYINYKK.M

R.HLGGLAIITR.S

K.FNPLTDKLK.D

K.SLFTVTPGSEQIR.A

K.EVYDFLASACAK.Y

K.KGEANSIVSSYNR.N

K.VGLIGSCTNSSYEDMSR.G + OM

R.GHLDNISNNMLIGAINEANGEANKVK.N + OM

11

E19 (-)

glyceraldehyde 3-phosphate dehydrogenase

ABV02551 (gi|157057025)

7.01/36.6

7.1/50.0

Penicillium marneffei

818

12/31

K.VGINGFGR.I

K.YDTQHGQFK.G

K.GTIEVEGSDLIVNGK.R

K.FYQERDPANIK.W

K.SSDTIISNASCTTNCLAPLAK.I

R.TAAQNIIPSSTGAAK.A

K.VIPALNGK.L

K.LTGMSMRVPTSNVSVVDLTCR.T

R.TEKPVSYDEIK.A

K.KYAEGELK.G

K.GIMGYTEDDVVSTDMNGNSNSSVFDAK.A + OM

K.AGIALNSNFIK.L

48

E26 (-)

60s ribosomal protein L2

XP_362607 (gi|39946140)

10.89/40.0

6.8/54.0

Magnaporthe grisea

132

3/3

K.DFLLPSNVVSQADLTR.L

R.LINSSEIQSVLR.A

R.LINSSEIQSVLRAPKGTAR.T

9

F46 (2)

enolase

Q76KF9 (gi|74662366)

5.14/47.3

6.0/58.1

Penicillium chrysogenum

850

11/35

K.LNQILR.I

K.VDEFLNK.L

K.SCNALLLK.V

K.KYDLDFK.N

K.IAMDVASSEFYK.E + OM

K.GVPLYAHISDLAGTK.K

K.WLTYEQLADLYK.S

K.VNQIGTLTESIQAAK.D

R.GNPTVEVDVVTETGLHR.A

K.TSDFQIVGDDLTVTNPLR.I

R.LAFQEFMIVPDTAPTFSEGLR.Q + OM

31

  1. a Spot, spot number corresponds to labeled spots in Figs. 2 and 3 (as indicated in parentheses); Protein name, matched protein description; NCBI Accession (Version), accession number and submission version of matched protein from NCBI database; Theo. pI/M r (kDa), theoretical isoelectric point and molecular mass based on amino acid sequence of the identified protein; Expt. pI/M r (kDa), experimental isoelectric point and molecular mass estimated from the 2DGE gels; Species, the fungal species of the matched protein; Mascot score, score obtained from the Mascot search for each match; NP, the number of matched peptides; PD, the number of peptides detected; MS/MS Peptide Sequence, amino acid sequences of peptides identified by Nano-LC/MS/MS; OM, oxidized methionine residue; SC, percent amino acid sequence coverage for the identified protein.