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Table 2 Identification of preferentially expressed proteins in the developing mould phase of P. marneffei.a

From: Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei

Spot (Fig.) Protein Name NCBI Accession (Version) Theo. pI/M r (kDa) Expt. pI/M r (kDa) Species Mascot Score NP/PD MS/MS Peptide Sequence SC (%)
B28 (2, 3) hypothetical protein (malate dehydrogenase, mitochondrial precursor) XP_001817504 (gi|169766066) 8.79/35.7 5.8/28.0 Aspergillus oryzae 591 8/12 R.VSELALYDIR.G R.IQFGGDEVVK.A R.LFGVTTLDVVR.A R.DDLFNTNASIVR.D K.RLFGVTTLDVVR.A K.DGAGSATLSMAMAGAR.M + 2 OM K.AKDGAGSATLSMAMAGAR.M + 2 OM K.VELGPNGVEKILPVGQVNAYEEK.L 25
C7 (-) hypothetical protein (S-adenosyl-L-homocysteine hydrolase) XP_658867 (gi|67521612) 6.02/49.3 5.7/60.5 Aspergillus nidulans 161 4/5 R.ATDVMIAGK.V + OM K.VAVVAGFGDVGK.G K.VADISLAAFGR.R K.SVQNIKPQVDR.Y 9
C18 (3) proteasome component Pre9 XP_750785 (gi|70991873) 5.80/28.5 5.7/49.1 Aspergillus fumigatus 165 4/5 K.IEFATVGK.T R.TTIFSPEGR.L K.LLEQDTSAEK.L K.LSSEKIEFATVGK.T 12
F47 (-) enolase AAK51201 (gi|13991101) 5.33/47.3 6.0/37.0 Penicillium citrinum 105 2/2 K.VNQIGTLTESIQAAK.D R.LAFQEFMIVPDTAESFSEGLR.Q 8
  1. a Spot, spot number corresponds to labeled spots in Figs. 2 and 3 (as indicated in parentheses); Protein name, matched protein description; NCBI Accession (Version), accession number and submission version of matched protein from NCBI database; Theo. pI/M r (kDa), theoretical isoelectric point and molecular mass based on amino acid sequence of the identified protein; Expt. pI/M r (kDa), experimental isoelectric point and molecular mass estimated from the 2DGE gels; Species, the fungal species of the matched protein; Mascot score, score obtained from the Mascot search for each match; NP, the number of matched peptides; PD, the number of peptides detected; MS/MS Peptide Sequence, amino acid sequences of peptides identified by Nano-LC/MS/MS; OM, oxidized methionine residue; SC, percent amino acid sequence coverage for the identified protein.