Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei

Table 2 Identification of preferentially expressed proteins in the developing mould phase of P. marneffei.^a

Spot (Fig.)	Protein Name	NCBI Accession (Version)	Theo. pI/M r (kDa)	Expt. pI/M r (kDa)	Species	Mascot Score	NP/PD	MS/MS Peptide Sequence	SC (%)
B28 (2, 3)	hypothetical protein (malate dehydrogenase, mitochondrial precursor)	XP_001817504 (gi\|169766066)	8.79/35.7	5.8/28.0	Aspergillus oryzae	591	8/12	R.VSELALYDIR.G R.IQFGGDEVVK.A R.LFGVTTLDVVR.A R.DDLFNTNASIVR.D K.RLFGVTTLDVVR.A K.DGAGSATLSMAMAGAR.M + 2 OM K.AKDGAGSATLSMAMAGAR.M + 2 OM K.VELGPNGVEKILPVGQVNAYEEK.L	25
C7 (-)	hypothetical protein (S-adenosyl-L-homocysteine hydrolase)	XP_658867 (gi\|67521612)	6.02/49.3	5.7/60.5	Aspergillus nidulans	161	4/5	R.ATDVMIAGK.V + OM K.VAVVAGFGDVGK.G K.VADISLAAFGR.R K.SVQNIKPQVDR.Y	9
C18 (3)	proteasome component Pre9	XP_750785 (gi\|70991873)	5.80/28.5	5.7/49.1	Aspergillus fumigatus	165	4/5	K.IEFATVGK.T R.TTIFSPEGR.L K.LLEQDTSAEK.L K.LSSEKIEFATVGK.T	12
F47 (-)	enolase	AAK51201 (gi\|13991101)	5.33/47.3	6.0/37.0	Penicillium citrinum	105	2/2	K.VNQIGTLTESIQAAK.D R.LAFQEFMIVPDTAESFSEGLR.Q	8

^a Spot, spot number corresponds to labeled spots in Figs. 2 and 3 (as indicated in parentheses); Protein name, matched protein description; NCBI Accession (Version), accession number and submission version of matched protein from NCBI database; Theo. pI/M r (kDa), theoretical isoelectric point and molecular mass based on amino acid sequence of the identified protein; Expt. pI/M r (kDa), experimental isoelectric point and molecular mass estimated from the 2DGE gels; Species, the fungal species of the matched protein; Mascot score, score obtained from the Mascot search for each match; NP, the number of matched peptides; PD, the number of peptides detected; MS/MS Peptide Sequence, amino acid sequences of peptides identified by Nano-LC/MS/MS; OM, oxidized methionine residue; SC, percent amino acid sequence coverage for the identified protein.

ISSN: 1477-5956