Table 3 Identification of preferentially expressed proteins in the developing yeast phase of P. marneffei.a
From: Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei
Spot (Fig.) | Protein Name | NCBI Accession (Version) | Theo. pI/M r (kDa) | Expt. pI/M r (kDa) | Species | Mascot Score | NP/PD | MS/MS Peptide Sequence | SC (%) |
|---|---|---|---|---|---|---|---|---|---|
A1 (2) | Ran GTPase spi1 | NP_596827 (gi|19113619) | 7.00/24.8 | 5.7/45.9 | Schizosaccharomyces pombe | 173 | 3/3 | R.HLTGEFEK.K K.LVLVGDGGTGK.T K.NLQYYDISAK.S | 13 |
A2 (2) | succinyl-CoA synthetase alpha subunit | DQ666366 (gi|110227027) | 5.88/27.7 | 7.0/54.4 | Penicillium marneffei | 321 | 5/5 | K.TLRDEFVR.R R.MGHAGAIVSGGK.G + OM K.IGIMPGFIHK.R + OM K.ISALEAAGVIVER.S K.AGTTHLDLPVFATVSDAVK.E | 23 |
A3 (2,3) | heat shock protein 30 | ABF82266 (gi|106647229) | 6.17/20.7 | 6.2/45.0 | Penicillium marneffei | 752 | 9/31 | R.SGDFAPLFR.L R.LLDDYDLHR.S R.DGQTPASSSISSFAPR.F K.DAYHLDGELPGIAQK.D K.DVEIEFSDPQTLTIK.G R.EYHTLPENENPHAPKPASVEDAPESSDETAVQ K.S R.SVGEFHR.S K.ASLKNGVLTVTVPK.A K.NGVLTVTVPK.A | 66 |
B23 (3) | NAD-dependent formate dehydrogenase | XP_001270055 (gi|121703582) | 7.65/46.2 | 6.5/57.4 | Aspergillus clavatus | 181 | 3/26 | K.NEYDLENK.V K.VVGTVAVGR.I K.VLMVLYDGGEHAK.Q + OM | 7 |
B38 (2) | antigenic mitochondrial protein HSP60 | EAL93225 (gi|71001164) | 5.53/62.1 | 5.6/61.8 | Aspergillus fumigatus | 1000 | 13/57 | K.FENLGAR.L K.LSGGVAVIK.V R.LLQDVASK.T K.AVTSTLGPK.G R.AIFSETVK.N R.GQLQVAAVK.A R.VVDALNATR.A K.APGFGDNRK.S K.VGGASEVEVGEK.K K.EDTIILNGEGSK.D K.NVAAGCNPMDLR.R + OM K.VEFEKPLILLSEK.K R.GVMADPSTSEYEKEK.L + OM | 22 |
B40 (-) | ATP synthase F1, subunit beta | XP_753589 (gi|70997705) | 5.30/55.6 | 4.2/47.0 | Aspergillus fumigatus | 1047 | 12/17 | K.IGLFGGAGVGK.T K.VVDLLAPYAR.G K.IHQVIGAVVDVK.F R.FTQAGSEVSALLGR.I K.AHGGYSVFTGVGER.T K.TVFIQELINNIAK.A K.VALVFGQMNEPPGAR.A + OM R.GISELGIYPAVDPLDSK.S R.FLSQPFTVAQVFTGIEGK.L R.EGNDLYHEMQETGVIQLEGESK.V + OM K.SLQDIIAILGMDELSEADKLTVER.A + OM K.GSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSR.G | 39 |
C25 (3) | Phospho-glycerate kinase | XP_752401 (gi|70995281) | 6.31/44.8 | 6.4/59.7 | Aspergillus fumigatus | 391 | 8/11 | K.TVGDIIEK.A R.IVGALPTIK.Y K.IQLIDNLLPK.V K.ASGGQVILLENLR.F R.AHSSMVGVDLPQK.A + OM K.YSLKPVVPELEK.L K.VSDKIQLIDNLLPK.V K.LSHVSTGGGASLELLEGK.E | 20 |
D7 (2) | hypothetical protein (activator of HSP90 ATPase) | EAS36198 (gi|90306567) | 5.11/36.5 | 6.1/56.1 | Coccidioides immitis | 164 | 2/7 | K.IVSMDGDVDVSQR.K + OM R.TTAEEMYTTFTDPQR.L | 8 |
D15 (-) | cell division control protein Cdc48 | XP_756045 (gi|71002728) | 5.09/90.5 | 5.1/67.4 | Aspergillus fumigatus | 221 | 3/3 | R.EVDIGIPDPTGR.L R.VVSQLLTLMDGMK.A + 2 OM R.VVNQLLTEMDGMTSK.K + 2 OM | 4 |
D16 (-) | heat shock protein 70 | AAX63812 (gi|62125797) | 5.03/69.6 | 5.1/64.0 | Penicillium marneffei | 1544 | 21/36 | R.TKDNNLLGK.F R.LSKEEIER.M K.EKLEAEIEK.T R.LVNHFASEFK.R K.FELTGIPPAPR.G K.FSDPEVQADAK.H K.DAGLIAGLNVLR.I R.IEIIANDQGNR.T K.YKAEDEAEATR.I R.KFSDPEVQADAK.H K.SSVHEIVLVGGSTR.I K.LVTDFFNKEPNK.S R.TTPSFVAFTDSER.L R.EADEVEERPEELD.- R.DDRIEIIANDQGNR.T K.QFTPEEISSMVLIK.M + OM K.ATAGDTHLGGEDFDSR.L K.NQVAMNPHNTVFDAK.R + OM R.IINEPTAAAIAYGLDKK.V K.SINPDEAVAYGAAVQAAILSGDTSSK.S R.GVPQIEVTFDMDANGIMNVSAVEK.G + 2 OM | 40 |
D18 (-) | hypothetical protein (UDP-N-acetylglucosamine pyrophos-phorylase) | XP_682363 (gi|67904214) | 6.03/56.0 | 5.9/61.2 | Aspergillus nidulans | 160 | 3/3 | K.SLFQLQAER.I R.REDEFSPLK.N R.NATESVGLILQK.N | 5 |
E7 (-) | adenylate kinase | XP_750499 (gi|70991300) | 7.71/28.9 | 6.3/44.8 | Aspergillus fumigatus | 206 | 3/6 | K.SIAEQMR.I + OM K.NGFILDGFPR.T K.IMDQGGLVSDEIMVNMIK.N + 3 OM | 13 |