Figure 1
From: Development of pan-specific antibody against trimethyllysine for protein research
Generation of anti-trimethyllysine antibody. A. The reaction scheme for chemical synthesis of KLH with trimethyllysine residues. B. The scheme for strategic affinity purification of the trimethyllysine-specific antibody. The antibody in serum cross-reacted to monomethyllysine and dimethyllysine is removed by the mMeK/dMeK affinity column. The remaining trimethyllysine-specific antibody in the serum is further purified using tMeK affinity column.