Figure 3
From: Development of pan-specific antibody against trimethyllysine for protein research
Western blot and immunoprecipitation analysis of proteins from human melanoma cells using the anti-tMeK antibody. A. Cells treated with or without TSA were lysed for Western blot (50 μg/lane) using the trimethyllysine-purified antibody, with and without the presence of free tMeK. B. Western blot analysis of the anti-tMeK immunoprecipitated proteins from the crude lysate of the mouse spleen using anti-tMeK HRP conjugates (0.25 μg/ml). The signal was competitively inhibited by the free tMeK (10 μg/ml). C. Western blot analysis of immunopreciptates by IgG control or anti-tMek antibody with H3K4me3, H3K9me3, or H3K27me3 antibodies.