Trypanosoma cruzi alkaline 2-DE: Optimization and application to comparative proteome analysis of flagellate life stages

Magalhães, Adriana D; Charneau, Sébastien; Paba, Jaime; Guércio, Rafael AP; Teixeira, Antonio RL; Santana, Jaime M; Sousa, Marcelo V; Ricart, Carlos AO

doi:10.1186/1477-5956-6-24

Proteome Science

Table 2 T. cruzi identified proteins and differential expression analysis among epimastigote and trypomastigote stages

From: Trypanosoma cruzi alkaline 2-DE: Optimization and application to comparative proteome analysis of flagellate life stages

Spot^a	Protein	Accession No. (NCBI)	MASCOT		MW	pI	Expression	E/T (SEM)^h	t-valueⁱ	P^j
			Seq. cov^b (%)	Score ^c	Exp (Theo) ^d	Exp (Theo) ^d
1	Alanine aminotransferase	gi\|71660439	16	76	55.7 (55.1)	6.2 (6.1)	E^e	-	-	-
2	Citrate synthase	gi\|71660323	13	76	53.6 (53.1)	8.0 (8.6)	E<T^g	0.35 ± 0.09	-7.144	0.019
3	Dehydrogenase	gi\|61741948	35	116	43.5 (42.4)	6.0 (6.1)	E^e	-	-	-
4	Dehydrogenase	gi\|61741944	29	81	43.9 (42.6)	6.2 (6.0)	E^e	-	-	-
5	D-isomer specific 2-hidroxyacid dehydrogenase	gi\|71420052	32	82	46.1 (38.8)	6.4 (6.0)	E>T^g	3.81 ± 0.10	27.755	0.001
6	Cystathionine beta-synthase	gi\|71425069	30	81	43.2 (42.8)	6.6 (6.4)	E<T^g	0.24 ± 0.03	-24.786	0.002
7	2-amino-3-ketobutyrate coenzyme A ligase	gi\|71650629	29	87	44.3 (44.0)	7.2 (6.7)	E = T^f	2.58 ± 0.55	2.897	0.101
8	Aspartate aminotransferase, mitochondrial	g\|71412236	22	87	43.3 (46.5)	7.7 (8.6)	E = T^f	1.00 ± 0.21	0.015	0.989
9	Arginine kinase	gi\|71407949	23	76	41.8 (40.5)	6.1 (6.3)	E = T^f	0.84 ± 0.10	-1.544	0.262
10	D-isomer specific 2-hidroxyacid dehydrogenase	gi\|71420052	47	140	42.0 (38.8)	6.4 (6.4)	E = T^f	5.97 ± 2.45	2.027	0.180
11	D-isomer specific 2-hidroxyacid dehydrogenase	gi\|71420052	42	99	41.9 (38.8)	6.6 (6.0)	E = T^f	1.77 ± 0.39	1.962	0.189
12	L-threonine 3-dehydrogenase	gi\|71406160	29	103	40.5 (37.3)	6.9 (6.7)	E>T^g	9.64 ± 1.65	5.228	0.035
13	Succinyl-CoA synthetase alpha subunit	gi\|71667777	29	81	40.9 (32.3)	7.8 (8.4)	E = T^f	0.91 ± 0.05	-1.762	0.220
14	Mitochondrial malate dehydrogenase	gi\|71414199	47	114	38.3 (31.9)	8.1 (7.6)	E = T^f	1.27 ± 0.13	2.022	0.181
15	Mitochondrial malate dehydrogenase	gi\|71414199	38	106	38.3 (31.9)	8.5 (7.6)	E = T^f	1.43	-	-
16	Pyridoxal kinase	gi\|71413338	19	76	33.1 (33.6)	6.0 (6.0)	E = T^f	4.10 ± 1.03	2.996	0.096
17	Tryparedoxin peroxidase	gi\|17224953	44	101	27.8 (22.7)	6.3 (6.0)	E = T^f	1.60 ± 0.51	1.173	0.361
18	Small GTP-binding protein	gi\|71413249	30	76	26.2 (22.0)	7.2 (7.0)	E = T^f	1.16 ± 0.34	0.479	0.679
19	Chain B, triosephosphate isomerase	gi\|4389145	26	123	30.0 (27.4)	8.3 (8.6)	E = T^f	0.64 ± 0.10	-3.543	0.071
20	Hypothetical protein	gi\|71414910	51	111	25.6 (21.5)	6.3 (6.9)	E^e	-	-	-
21	Peptide methionine sulfoxide reductase	gi\|71405176	60	110	22.7 (20.2)	6.3 (6.1)	E = T^f	4.10 ± 1.02	3.040	0.093
22	Cyclophilin A	gi\|71659715	34	85	21.1 (19.0)	7.8 (8.4)	E = T^f	3.20 ± 1.52	1.448	0.285
23	Cyclophilin A	gi\|71659715	37	82	20.8 (19.0)	8.4 (8.4)	E = T^f	1.28 ± 0.28	0.994	0.425
24	Cyclophilin A	gi\|71659715	41	80	19.3 (19.0)	8.6 (8.4)	E = T^f	1.28 ± 0.26	1.071	0.396
25	Calpain-like cystein peptidase	gi\|71407848	51	97	17.1 (15.1)	6.6 (6.4)	E^e	-	-	-
26	Nucleoside diphosphate kinase	gi\|71667532	54	94	17.3 (17.0)	8.5 (8.5)	E = T^d	3.75 ± 2.04	1.346	0.311
27	20 kDa heat shock protein	gi\|71418782	42	78	16.6 (16.0)	7.8 (7.8)	E^e	-	-	-
28	Hypothetical protein	gi\|71407758	52	104	16.0 (13.0)	7.7 (7.8)	E^e	-	-	-
29	10 kDa heat shock protein	gi\|71410853	49	78	14.8 (10.7)	6.4 (8.0)	E = T^f	1.67 ± 0.39	1.729	0.334
30	69 kDa Paraflagelar rod protein	gi\|71650963	16	80	71.0 (70.1)	6.0 (5.9)	T^e	-	-	-
31	Trans-sialidase	gi\|71409133	11	88	75.0 (81.4)	7.0 (8.4)	T^e	-	-	-
32	Hypothetical protein	gi\|71651158	47	87	13.1 (16.4)	9.6 (9.0)	T^e	-	-	-

^aSpot numbers correspond to those indicated in Fig. 2 and Fig. 3
^bPercentage of predicted protein sequence covered by matched peptides via Mascot
^cProbability Based Mowse Score of Mascot software
^dTheoretical Mr and pI calculated from amino acid sequence
^eSpots specifically detected in epimastigote (E) or trypomastigote (T)
^fProtein expression not statistically different between both life stages by t-test (p < 0.05)
^gStatistically significant (t-test, p < 0.05) increased spot volume in epimastigote (E>T) and trypomastigote (E<T)
^hEpimastigote/trypomastigote (E/T) spot volume ratio with standard error of the mean (SEM)
ⁱDecision value of the t-test
^jProbability value that E/T ratio is different from 1 in a random event

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ISSN: 1477-5956

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