Figure 2
Improved resolution with zoom-in 2-D gels. Details of the 2-D gel zoomed areas. We showed the improved separation and visualization of the mouse serum proteome. In fact, multiple isoforms for most identified proteins were found and other identified spots were detected only in 4–7 pH-range and not in 3–10 pH-range. Panel 1: 3–10 pH range (1 spot 1 id: mouse ceruloplasmin; 4 spots 1 id: mouse gelsolin) 4–7 pH range (14 spots 3 ids: mouse ceruloplasmin, mouse alpha-macroglobulin, mouse albumin; 6 spots 1 id: mouse gelsolin; 3 spots 1 id: mouse hemopexin).Panel 2: 3–10 pH range (1 smear 2 ids: mouse afamin and mouse hemopexin; 1 smear 1 id: mouse kininogen; 1 smear 2 ids: mouse antithrombin-III, mouse Alpha-2-HS-glycoprotein) 4–7 pH range (3 spots 1 id: mouse prothrombin; 3 spots 1 Id: mouse hemopexin; 3 spots 1 id: mouse kininogen; 6 spots 4 ids: mouse antithrombin-III, mouse Alpha-2-HS-glycoprotein, mouse vitamin D-binding protein and mouse fetuin-b).Panel 3: 3–10 pH range (3 spots 2 ids: mouse apolipoprotein A4 and mouse zinc-alpha-2-glycoprotein; 1 spot 1 id: mouse albumin) 4–7 pH range (1 spot 1 id: mouse serum paraoxonase/arylesterase 1; 1 spot 1 id: mouse H-2 class I histocompatibility antigen; 3 spots 1 id: mouse alpha-2-macroglobulin). Panel 4: 3–10 pH range (3 spots 1 id: mouse apolipoprotein A1) 4–7 pH range (1 spot 1 id: mouse mannose-binding protein2; 4 spots 1 id: mouse Ig kappa chain V-III region; 2 spots 1 id: mouse apolipoprotein A2). The spots 1 (alpha-2-macroglobulin); 2 (complement C1r-subcomponent); 3 and 3a (Apoliprotein A4 and Zinc-alpha-glycoprotein 2 respectively) and 4 (glutathione peroxidase 3) are examples discussed respectively in the text and in Figure 5.