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Table 1 Mouse serum protein map

From: A simple and reliable protocol for mouse serum proteome profiling studies by use of two-dimensional electrophoresis and MALDI TOF/TOF mass spectrometry

No.

SwissProt entry

SwissProt accession

Mw (KDa)

pI

Mascot score

sequence coverage (%)

msms (m/z)

matrix

Our Map 1 (matched peptides)

Ref Map 2 (matched peptides)

Ref Map 3 (matched peptides)

Ref Map 4 (matched peptides)

Ref Map 5 (matched peptides)

Ref Map 6 (matched peptides)

1

A1AG1 MOUSE

Q60590

24

5.6

124

32

1703.91

CHCA DHB

10

not present

4

12

5 (up regulated)

present

2

AMBP MOUSE

Q07456

40

6

38

4

1669.85

CHCA

1

3*

not present

not present

not present

not present

3

APOM MOUSE

Q9Z1R3

21.6

6

105

36

938.42

CHCA DHB

9

not present

not present

not present

not present

present

4

MUP1 MOUSE

P11588

20.9

5

115

55

 

DHB

12

0

10

not present

not present

not present

 

MUP2 MOUSE

P11589

20.9

5

146

68

 

DHB

14

0

11

19

not present

not present

 

MUP6 MOUSE

P02762

20.9

5

136

62

 

DHB

12

0

10

not present

not present

not present

 

MUP8 MOUSE

P04938

17.7

5

141

74

 

DHB

12

0

10

not present

not present

not present

5

A1AT1 MOUSE

P07758

46.1

5.4

145

41

981.56

1137.66

1232.70

2003.04

2327.09

2405.17

3498.80

CHCA DHB

15

10*

2'

13

12

20 (up regulated)

not present

 

A1AT2 MOUSE

P22599

46.1

5.3

104

36

2405.17

2003.04

2327.09

3498.80

CHCA DHB

12

9'

not present

7

3 (up regulated)

present

 

A1AT3 MOUSE

Q00896

46

5.3

146

44

981.56

1137.66

2003.04

2327.09

2405.17

3498.80

CHCA DHB

15

not present

16

15

not present

not present

 

A1AT4 MOUSE

Q00897

46.1

5.2

162

51

1232.74

2003.04

2327.09

3498.80

CHCA DHB

17

not present

not present

not present

not present

present

 

A1AT5 MOUSE

Q00898

46

5.4

105

35

1232.71

2327.09

2405.17

CHCA DHB

12

not present

not present

not present

not present

present

 

A1AT6 MOUSE

P81105

46

5.2

161

47

981.56

1137.66

1232.70

2003.04

2327.09

2405.17

3498.80

CHCA DHB

16

2

not present

8

not present

present

6

ANT3 MOUSE

P32261

52.5

6.1

298

56

1198.70

1340.69

1359.67

1700.89

CHCA DHB

29

6

not present

not present

not present

present

7

A2AP MOUSE

Q61247

55.1

5.8

169

36

1591.80

1680.81

CHCA DHB

15

3

not present

not present

not present

present

8

CBG MOUSE

Q06770

44.9

5

148

32

 

CHCA DHB

10

3

not present

not present

not present

present

9

SPA3K MOUSE

P07759

47

5

113

29

 

CHCA DHB

12

12

not present

21

7 (up regulated)

present

10

A2MG MOUSE

Q61838

167

6.3

153

19

2068.21

CHCA DHB

18

25

16 (immuno depletion)

not present

not present

present

10a

A2MG MOUSE C-term

Q61839

28

7

62

6

1031.50

1111.58

1216.61

1787.96

CHCA DHB

9

?

not present

13

16 (up regulated)

present

11

ADIPO MOUSE

Q60994

26.9

5.3

63

19

1504.72

DHB

5

not present

not present

not present

not present

present

12

AFAM MOUSE

O89020

71.5

5.5

155

31

 

CHCA DHB

15

1

9

not present

not present

present

13

ALBU MOUSE

P07724

70.7

5.7

426

68

1455.83

1479.70

1609.90

1882.96

1960.15

1981.98

CHCA DHB

40

26*

46'

17

48

12 (up regulated)

present

14

ALS MOUSE

P70389

67.7

6.1

97

28

 

DHB

13

not present

not present

not present

not present

present

15

CPN2 MOUSE

Q9DBB9

61.3

5.5

131

25

 

CHCA

10

not present

not present

not present

not present

present

16

ANGL6 MOUSE

Q8R0Z6

51.4

9.2

56

16

 

DHB

4

not present

not present

not present

not present

not present

17

ANXA2 MOUSE

P07356

38.8

7.5

82

18

 

CHCA

5

not present

not present

not present

not present

not present

18

APOA1 MOUSE

Q00623

30.5

5.6

207

49

1237.67

1318.61

1331.53

1340.77

CHCA DHB

17

5*

18'

13

18

22

present

19

APOA2 MOUSE

P09813

11.3

6.6

85

14

1193.62

1831.98

CHCA DHB

2

4'

7

3

3 (up regulated)

present

20

APOA4 MOUSE

P06728

45

5.4

223

60

1131.66

1231.61

1461.75

2023.05

CHCA DHB

21

2*

5'

15

29

6 (up regulated)

present

21

APOE MOUSE

P08226

35.9

5.6

166

51

968.52

1075.60

1599.82

CHCA DHB

21

5'

7

17

16 (up regulated)

present

22

APOC3 MOUSE

P33622

10.9

4.6

140

19

1062.46

1078.45

1987.94

CHCA

3

not present

3

3

not present

present

23

APOH MOUSE

Q01339

39.9

8.6

276

62

1325.68

1544.85

2719.48

CHCA DHB

22

7

not present

17

22 (up regulated)

present

24

CLUS MOUSE

Q06890

55.2

5.5

103

19

 

CHCA DHB

11

2*

9

12

not present

present

25

C1R MOUSE

Q8CG16

81.5

5.4

78

17

 

CHCA

8

not present

not present

not present

not present

not present

26

CFAB MOUSE

P04186

86.3

7.2

269

37

 

CHCA DHB

23

3

not present

not present

not present

present

27

CFAI MOUSE

Q61129

69.5

7.4

103

21

1726.85

DHB

11

3

not present

not present

not present

not present

28

HGFA MOUSE

Q9R098

72.9

6.6

58

13

 

DHB

6

not present

not present

not present

not present

present

29

HPT MOUSE

Q61646

39.2

5.9

146

49

980.49

1320.74

1373.61

DHB

21

not present

11

5

14-3 (up and down regulated)

present

29a

HPT MOUSE

Q61646

  

81

11

1679.78

DHB

6

?

?

14

?

not present

30

PLMN MOUSE

P20918

93.4

6.2

387

53

1138.46

CHCA DHB

35

8

not present

32

6

present

31

CFAH MOUSE

P06909

144

6.6

269

31

 

CHCA DHB

29

6

not present

18

not present

present

32

CS1A MOUSE

Q8CG14

78.3

5

66

15

 

DHB

6

not present

not present

not present

not present

not present

33

F13B MOUSE

Q07968

78.3

5.6

148

26

 

CHCA

15

not present

not present

not present

not present

present

34

CO3 MOUSE

P01027

188

6.4

312

29

1886.93

CHCA DHB

40

23*

1'

not present

not present

17 (up regulated)

present

35

CO4 MOUSE

P01029

194

7.5

123

9

 

CHCA

14

3

not present

not present

not present

present

36

CO9 MOUSE

P06683

63.2

5.6

80

29

 

DHB

14

1

not present

not present

not present

not present

37

C1QB MOUSE

P14106

27

8.6

69

20

 

CHCA

5

1*

not present

not present

not present

present

38

EGFR MOUSE

Q01279

138

6.5

171

16

 

CHCA DHB

15

not present

not present

18

not present

present

39

FIBB MOUSE

Q8K0E8

55.4

6.7

112

39

 

CHCA DHB

20

not present

not present

not present

not present

present

40

FINC MOUSE

P11276

276

5.4

90

8

 

CHCA

16

9*

not present

not present

not present

present

41

FCN1 MOUSE

O70165

36.8

6

65

12

 

DHB

5

not present

not present

8

not present

not present

42

FETUA MOUSE

P29699

38.1

6

135

47

1653.75 2138

CHCA DHB

11

3

6

8

not present

present

43

FETUB MOUSE

Q9QXC1

43.5

6.2

113

30

1382.89

1159.7

CHCA DHB

14

1

5

13

not present

present

44

GPX3 MOUSE

P46412

25.6

8.3

98

45

1955

CHCA DHB

12

not present

not present

not present

not present

present

45

HA10 MOUSE

P01898

37.2

5.2

173

46

1671.86

CHCA DHB

16

3

not present

not present

not present

not present

46

HBA MOUSE

P01942

15

8

79

31

1589.82

1819.93

CHCA

5

2*

6'

not present

not present

8

present

47

HBB1 MOUSE

P02088

16

7.3

68

73

1274.72

CHCA DHB

9

not present

not present

not present

7 (up regulated)

present

48

HEMO MOUSE

Q91X72

52

7.9

188

43

1100.47

1212.63

1504.76

1516.71

1727.77

2472.12

CHCA DHB

21

14

8

25

3 (up regulated)

present

49

IGHG1 MOUSE

P01868

36.2

7.2

94

45

 

DHB

9

3

not present

not present

not present

not present

50

KNG1 MOUSE

O08677

74.1

6

164

29

1010.56

1060.56

1515.68

CHCA DHB

18

9

12

24

5 (up regulated)

not present

51

KLKB1 MOUSE

P26262

73.4

8.4

105

22

 

CHCA

14

1*

2

not present

not present

present

52

KV3A1/2/4 MOUSE

P01654

12

5

116

16

1616.89

1855.04

CHCA DHB

2

not present

not present

not present

not present

present

 

KV3AD MOUSE

P01665

12

4.9

54

44

1155.46

1616.89

1855.04

CHCA DHB

4

not present

not present

not present

not present

present

53

KV5AB MOUSE

P01644

12

7.9

88

17

1028.56

1926.92

2455.35

CHCA DHB

3

not present

not present

not present

not present

not present

 

KV5J MOUSE

P01645

11.9

9.2

93

16

1926.81

CHCA DHB

1

not present

not present

not present

not present

present

54

KV3N MOUSE

P01666

12

4.5

58

53

 

DHB

3

not present

not present

not present

not present

present

55

MUC MOUSE

P01872

50

6.6

184

30

1330.78

1603.91

CHCA DHB

14

4

not present

not present

not present

not present

56

MUG1 MOUSE

P28665 (Q80XE6)

166.4

6

168

20

 

CHCA DHB

24

12

not present

not present

not present

present

57

PHLD MOUSE

O70362

93.8

6.6

151

23

 

CHCA DHB

20

1

not present

not present

not present

present

58

PON1 MOUSE

P52430

34.6

5

117

32

1853.9

CHCA DHB

8

2

not present

not present

not present

present

59

PROP MOUSE

P11680

50

8.4

87

21

 

CHCA

8

not present

not present

not present

not present

present

60

RETBP MOUSE

Q00724

23.5

5.7

138

59

1226.63

1360.58

1789.84

2079.88

CHCA DHB

15

1

not present

not present

not present

present

61

SAMP MOUSE

P12246

26.4

6

88

38

2133.03

CHCA DHB

8

1

8

8

not present

present

62

THRB MOUSE

P19221

71.6

6

156

28

1189.56

CHCA DHB

19

4

not present

26

9 (up regulated)

present

63

TRFE MOUSE

Q921I1

78.8

7

308

51

1171.61

1419.86

1656.81

1990.82

2007.94

CHCA DHB

38

39*

1'

?

26

not present

present

64

KAC MOUSE

P01837

11.9

5

91

87

990.51

CHCA DHB

8

1

not present

not present

not present

not present

65

ZA2G MOUSE

Q64726

35.4

5.8

173

55

1274.60

1318.81

1409.72

1610.74

CHCA DHB

15

1

not present

19

not present

present

66

VTDB MOUSE

P21614

55.1

5.4

266

44

1051.6

1303.77

2441.13

CHCA DHB

24

3

12

31

not present

present

67

TTHY MOUSE

P07309

15.9

5.8

122

67

1382.62

1554.89

2438.17

2517.22

CHCA DHB

8

4*

1'

10

9

not present

present

68

GELS MOUSE

P13020

86.3

5.8

233

39

1254.75

1275.73

CHCA DHB

22

6

not present

19

not present

present

69

VTNC MOUSE

P29788

55.6

5.7

82

23

 

CHCA DHB

10

2

not present

not present

not present

present

70

GCAB MOUSE

P01864

37

8.5

31.5

5

1913.84

DHB

1

2 (membrane form)

not present

not present

not present

not present

71

GCB MOUSE

P01866

37.3

7.2

79

30

1778.87

CHCA DHB

8

3 (membrane form)

not present

not present

not present

not present

72

LAC1 MOUSE

P01843

11.7

5.9

70

61

 

DHB

4

not present

not present

not present

not present

not present

73

LAC2 MOUSE

P01844

11.4

5.9

70

89

 

DHB

5

not present

not present

not present

not present

present

74

ACTG MOUSE

P63260

42.1

5.3

188

53

1198.75

1790.93

1954.14

CHCA DHB

16

not present

not present

not present

not present

not present

 

ACTB MOUSE

P60710

42

5.3

171

49

1198.75

1790.93

1954.14

CHCA DHB

15

not present

not present

not present

not present

present

75

APC MOUSE

Q61315

313

7.4

53

6

 

CHCA

15

not present

not present

not present

not present

present

76

CT160 MOUSE

Q8VCC6

22

9.5

56

20

 

CHCA

5

not present

not present

not present

not present

not present

77

CUL1 MOUSE

Q9WTX6

90.3

8.2

62

15

 

CHCA

7

not present

not present

not present

not present

not present

78

ESTN MOUSE

P23953

61.4

5.1

168

37

911.45

CHCA DHB

21

12

7

not present

not present

present

79

ITIH2 MOUSE

Q61703

106

6.8

150

24

1337.73

CHCA DHB

14

2

not present

not present

not present

present

80

ITIH4 MOUSE

9055252

104.8

6

133

20

 

CHCA

17

not present

not present

18

not present

not present

81

K2C5 MOUSE

Q922U2

62

7.6

60

16

 

DHB

11

not present

not present

not present

not present

not present

82

LIFR MOUSE

P42703

123.8

5.7

151

19

 

DHB

15

not present

not present

22

not present

present

83

MBL1 MOUSE

P39039

25.8

7.5

100

27

1544.89

CHCA

9

not present

not present

not present

8 (up regulated)

present

84

MBL2 MOUSE

P41317

26.3

5

70

29

1323.60

1522.72

1766.83

CHCA DHB

6

2

not present

8

not present

not present

85

OST5 MOUSE

Q8BSL4

40.7

9.7

70

23

 

CHCA

6

not present

not present

not present

not present

not present

86

SYT2 MOUSE

P46097

47.7

8.2

61

19

 

CHCA

6

not present

not present

not present

not present

present

87

TPH1 MOUSE

P17532

51.9

6

59

17

 

CHCA

7

not present

not present

not present

not present

not present

88

KPYM MOUSE

P52480

58

7

53

30

 

DHB

12

not present

not present

not present

not present

not present

89

DYN2 MOUSE

P39054

98

7

68

12

 

CHCA DHB

9

not present

not present

not present

not present

not present

90

GAB2 MOUSE

Q9Z1S8

73.6

8.5

33

2

1794.8

DHB

1

not present

not present

not present

not present

present

  1. Mouse serum proteins identified by us in serum from C57BL6 mice (Our Mapping 1), in comparison with previous mouse serum maps (Ref Map 2: [9], Ref Map 3: [8], Ref Map 4: [7, 32], Ref Map 5: ref [33] and Ref Map 6: [6]). Exactly, 16 of them (italic font) were identified only by narrow IPGs of pH 4–7. In bold, we highlighted the proteins identified only with LIFT-MS/MS measurements. In case of TCA/methanol precipitation method (Ref Map 2: [9]) we specified where the proteins were identified. (n*: precipitated fraction; n': supernatant fraction-). In the case of MUPs, we marked with 0 the entries, because there are evidences that those proteins are present just in C57BL6 mice and not in BALA/cj inbred strain [32].