1D Electrophoresis. 1D electrophoresis (fourth dimension fractionation) showing differential expression of protein band above 62 kDa m.w. marker. Hemopexin was identified as the most prominent protein in this band by LCMS/MS sequencing. Equal amount of protein (based on absorbance at 214 nm) from each sample was loaded per lane. Gel was stained with SyproRuby.