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Table 1 Summary of fractions selected for mass spectrometry analyses.

From: Multidimensional protein fractionation using ProteomeLab PF 2D™ for profiling amyotrophic lateral sclerosis immunity: A preliminary report

Fraction ID**
(Fraction Coordinates)
Patient 1 Patient 2 Patient 3
  GA Immunization
  Before
(B)
After
(A)
Before
(B)
After
(A)
Before
(B)
After
(A)
F1
(pH: 5.90-5.60, RT:16.04–16.71)
1.49* 1.11 1.76 1.49 1.41 1.46
F2
(pH: 7.10-6.80, RT:14.03–14.70)
0.004 0.006 0.023 0.009 0.008 0.009
F3
(pH: 6.20-5.90, RT:14.70–15.37)
0.043 0.049 0.06 0.049 0.03 0.031
F4
(pH: 6.50-6.20, RT:16.04–16.71)
0.28 0.343 0.57 0.533 0.23 0.11
F5
(pH: 5.90-5.60, RT:16.71–17.38)
0.178 0.171 0.507 0.24 0.134 0.133
F6
(pH: 6.20-5.90, RT:15.37–16.04)
0.01 0.02 0.1 0.09 0.06 0.03
F7
(pH: 4.99-4.88, RT:14.70–15.37)
0.24 0.49 0.48 0.805 0.51 0.46
F8
(pH: 6.80-6.50, RT:15.37–16.04)
0.002 0.016 0.054 0.04 0.01 0.005
F9
(pH: 6.80-6.50, RT:16.04–16.71)
0.11 0.03 0.045 0.065 0.02 0.01
F10
(pH: 6.80-6.50, RT:14.03–14.70)
0.005 0.006 0.024 0.010 0.0086 0.009
  1. Second dimension fractions representing selected pH ranges and retention times (RT). Corresponding peak heights are listed as absorbance units at 214 nm (*). Although there may be several peaks/fraction, values represent the peak having the greatest absorbance in the corresponding fraction. Fraction IDs are used in Table 2 (**).