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Table 1 Summary of fractions selected for mass spectrometry analyses.

From: Multidimensional protein fractionation using ProteomeLab PF 2D™ for profiling amyotrophic lateral sclerosis immunity: A preliminary report

Fraction ID**

(Fraction Coordinates)

Patient 1

Patient 2

Patient 3

 

GA Immunization

 

Before

(B)

After

(A)

Before

(B)

After

(A)

Before

(B)

After

(A)

F1

(pH: 5.90-5.60, RT:16.04–16.71)

1.49*

1.11

1.76

1.49

1.41

1.46

F2

(pH: 7.10-6.80, RT:14.03–14.70)

0.004

0.006

0.023

0.009

0.008

0.009

F3

(pH: 6.20-5.90, RT:14.70–15.37)

0.043

0.049

0.06

0.049

0.03

0.031

F4

(pH: 6.50-6.20, RT:16.04–16.71)

0.28

0.343

0.57

0.533

0.23

0.11

F5

(pH: 5.90-5.60, RT:16.71–17.38)

0.178

0.171

0.507

0.24

0.134

0.133

F6

(pH: 6.20-5.90, RT:15.37–16.04)

0.01

0.02

0.1

0.09

0.06

0.03

F7

(pH: 4.99-4.88, RT:14.70–15.37)

0.24

0.49

0.48

0.805

0.51

0.46

F8

(pH: 6.80-6.50, RT:15.37–16.04)

0.002

0.016

0.054

0.04

0.01

0.005

F9

(pH: 6.80-6.50, RT:16.04–16.71)

0.11

0.03

0.045

0.065

0.02

0.01

F10

(pH: 6.80-6.50, RT:14.03–14.70)

0.005

0.006

0.024

0.010

0.0086

0.009

  1. Second dimension fractions representing selected pH ranges and retention times (RT). Corresponding peak heights are listed as absorbance units at 214 nm (*). Although there may be several peaks/fraction, values represent the peak having the greatest absorbance in the corresponding fraction. Fraction IDs are used in Table 2 (**).