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Figure 4 | Proteome Science

Figure 4

From: Improved proteome coverage by using iTRAQ labelling and peptide OFFGEL fractionation

Figure 4

Analysis of SH-SY5Y peptides pI after OFFGEL fractionation and MALDI- MS/MS identification. (A) The average experimental pH of all peptides in a single fraction after filtering for false positive is presented as bars; white bars: non-labelled peptides; dark bars: iTRAQ peptides. Error bars indicate the SD of each fraction's experimental pI. The broken line is based on the theoretical pI values for an IPG strip of 24 cm ranging from pH 3–10; Agilent Technologies provided the theoretical pI values. (B) The total bar height shows the number of peptides in each fraction after filtering for false positive; white bars: non-labelled peptides; black bars: iTRAQ peptides.

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