Table 1 The list of experiments and the number of identified proteins
From: Improved proteome coverage by using iTRAQ labelling and peptide OFFGEL fractionation
Experiment | Quantity (μg) | Fractionation | Labelling | Total proteins | Proteins with 2 peptides (at least) | Yield (%) |
|---|---|---|---|---|---|---|
NL-116 | 200 | SCX | no | 159 | 116 | 73 |
NL-184 | 200 | OFFGEL-IEF | no | 285 | 184 | 64 |
NL-235 | 200 | SCX + OFFGEL-IEF | no | 302 | 235 | 78 |
iTRAQ-310 | 200 | SCX | iTRAQ | 472 | 310 | 66 |
iTRAQ-429 | 400 | SCX | iTRAQ | 492 | 429 | 87 |
iTRAQ-739 | 400 | OFFGEL-IEF | iTRAQ | 879 | 739 | 84 |