Proteome profiling of embryo chick retina

Table 1 Differentially expressed proteins identified by proteomics from retinas of ED7, ED11 and ED15 of embryo chick retina.

						Theoretical value		Experimental value		Changes(ratio)
	Protein	Est'Z	Taxonomy	Sequence coverage(%)	ncbi ID	pH	Mr (kDa)	pH	Mr (kDa)	ED7:ED7:ED15
S1	Fatty acid-binding protein, retina	2.13	Chick	61	Q05423	5.6	15.02	5.5	15	(-):(+):(+)
S2	Wnt-9	0.98	P. hagfish	37	P28124	11.3	15.06	5.75	10	1:14:4.6
S3	Ubiquitin like protein, SMT3A	0.4	Human	24	P55854	5.3	11.68	7	10	1:9.6:1.2
S4	unnamed protein product	0.66	Mouse	12	BAC32207.1	5.3	80.05	6.75	70	1:2.4:2.3
S5	Alpha enolase	2.38	Chick	35	P51913	6.2	47.63	6.5	100	1:2.2:1.7
S6	Sin3B	0.76	Human	10	AAH05113	6.6	49.62	9	45	2.1:1:0.3
S7	hypothetical protein	1.03	Human	23	CAD38885.1	9.2	26.71	9	35	2.5:1:1.7
S8	unnnamed protein product	0.99	Human	44	BAB14762.1	5.5	29.35	4	20	2.4:1:1.7
S9	unnamed protein product	1.51	Human	43	BAB85061.1	5.4	17.7	4	20	4.2:1:1.6
S10	ZZ type zinc finger containing protein	0.52	C. elegans	15	NP498699.1	4.5	64.85	4.5	30	7.5:1:4.2
S11	GABA receptor-A beta-3	0.61	Chick	12	P19019	9.4	54.76	5	45	2.8:1:4.1
S12	Flice	0.72	Mouse	18	CAA04196.1	5.3	49.35	5	70	1:2.9:(-)
S13	Keratin-9	2.43	Human	36	NP000217.1	5.1	62.2	9	45	1:2.3:4.0

S1 – S13 means ID numbers of spots. Taxonomy category was used for search. Probability, Est'Z, sequence coverage, and theoretical value of pI and Mr were obtained from the Pro Found search. The calculation of experimental pI and Mr was based on the migration of the protein in a 2D gel. Changes indicate the ED7 chick retinal protein volume ratio compared with that of ED11 and ED15. (-) indicate that protein spots were not detected

ISSN: 1477-5956