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Figure 2 | Proteome Science

Figure 2

From: Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

Figure 2

(A-D): A: The partitioning of the proteins between RIPA and urea buffers in the infiltrating Ductal Carcinoma case T2-018T (TUMOR). Venn diagram showing that RIPA and urea lysis buffers fractionate breast tumor proteins primarily on the basis of molecular weights. The average molecular weight of the 217 proteins that dissolve exclusively in RIPA buffer is 61604 m/z, whereas the 73 proteins that dissolve exclusively in urea buffer have an average molecular weight of 99154 m/z. B: Differential partitioning of proteins extracted from the T2-048 TUMOR between RIPA and urea buffers. Venn diagram showing that RIPA and urea lysis buffers fractionate breast tumor proteins on the basis of molecular weights. The average molecular weight of the 69 proteins that dissolve almost exclusively in RIPA buffer is 72330 m/z, whereas the 187 proteins that dissolve exclusively in urea buffer have an average molecular weight of 79704 m/z. Seventy-four proteins, whose average molecular weight is 47854m/z, dissolve fully in both RIPA and urea buffers. C: Matched normal breast tissue T2-048 NORMAL between RIPA and UREA buffers. The average molecular weight of the 128 proteins that are soluble nearly exclusively in RIPA buffer is 61879 m/z, whereas the 55 proteins that dissolve exclusively in urea buffer have an average molecular weight of 93810 m/z. Sixty-seven proteins with an average molecular weight of 53102m/z are fully soluble in both RIPA and urea buffers. D: Bilateral Adenocarcinoma case T2-029 TUMOR. The average molecular weight of the 104 proteins that dissolve almost exclusively in RIPA buffer is 51485 m/z, whereas the 247 proteins that dissolve exclusively in urea buffer have an average molecular weight of 76092 m/z. One hundred and eleven proteins with an average molecular weight of 45126 m/z are fully soluble in both RIPA and urea buffers.

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