Figure 8From: Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers Extraction of proteins from breast tumors for proteomics. proteins of the extracellular region (A) and cytoskeleton (B) are more soluble in urea buffer than in RIPA, whereas for cytoplasmic (C) and mitochondrial (D) proteins, RIPA buffer is preferred.Back to article page