Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

Table 1 Characteristics of the breast tumors and matched normal breast tissues analyzed in this work.

	T2-018 Tumor	T2-048 Tumor	T2-048 Normal	T2-029 Tumor
Location	Right breast	Left breast	Left breast	Bilateral
Diagnosis	Infiltrating ductal carcinoma	Infiltrating ductal carcinoma	Normal	Adenocarcinoma
Stage	IV T4N0M0	IIB T2N1M0	Normal	IV T4N1M0
Grade	II	I	I	III
Sex	Female	Female	Female	Female
Age	75 years	39 years	39 years	47 years
Gross Findings	Tumor size: 8 × 8 cm., ill demarcated. Cut section firm	Tumor size: 3 × 3.5 cm. ill demarcated. Cut section soft and white.	Tissue size: 3 × 3.5 cm.	Tumor size 2 × 2 cm., and 3 × 2 cm., ill demarcated. Cut section firm and gray/white

Source: Integrated Laboratory Services-Biotech (ILSbio), Chestertown, MD 21620. http://www.ilsbio.com ILS-19106.
Detailed histopathology reports are available at the Protein BioTechnologies website : http://www.proteinbiotechnologies.com/

ISSN: 1477-5956