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Figure 3 | Proteome Science

Figure 3

From: Label-free detection of biomolecular interactions in real time with a nano-porous silicon-based detection method

Figure 3

Nano-porous silicon immobilization chemistries. Top row: Carboxyl chemistry. Carboxyl functionalized nano-porous silicon chips (1) are activated and functionalized with EDC/sulfo-NHS. The sulfo-NHS ester functionalized surface (2) will react with amines contributed by the protein to be immobilized (3), leading to the formation of a stable amine bond between protein and surface (4). Bottom row: The protein to be immobilized is reacted with the bifunctional HyNic reagent (1), which will react with amines contributed from the protein to form hydrazine-functionalized protein. The hydrazine-functionalized protein is reacted with benzaldehyde functionalized nano-porous silicon surface (2), which will lead to a stable attachment of the protein to the surface through a hydrazone bond (3).

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