Figure 4

Typical full immobilization and binding run with non-specific binding test using a benzaldehyde biochip and hydrazone cross-linking chemistry. The signal shown is the result of subtracting the sample channel from the reference from the differential flow cells used with Ski Pro. 50 μL sample and reference volumes each were exposed to the sensor surface over 500 seconds. We applied 0.5 mg/mL HyNic-labeled streptavidin in the sample channel and conjugation buffer in the reference channel for 500 seconds, followed by 0.1 mg/mL biotinylated BSA. 10% delipidated rat plasma was run as a non-specific binding control.