Figure 5

Non-specific binding properties of benzaldehyde-functionalized nanoporous silicon surface. Increasing concentrations of rat plasma, diluted in PBS buffer with 0.05 g/L citrate, were applied to a chip, using PBS as reference. Application of 10% (v/v) diluted plasma does not lead to an appreciatable amount of binding, while 20% (v/v) and 40% (v/v) rat plasma lead to a temporary signal during sample application. After exposure to complex plasma mixtures, the chip surface remains competent for specific binding with HyNic-activated streptavidin (SA). The negative signal in 0% (v/v) and 10% (v/v) rat plasma is likely due to buffer effects. 50 μL sample and reference volumes each were exposed to the sensor surface over 500 seconds.