Figure 9

Comparison of SKiPro and Biacore 3000, using EDC/S-NHS chemistry. An 18 OPD nanoporous silicon carboxyl chip (SKi Pro) and a 4000 RU CM-5 chip (Biacore) were prepared by immobilizing human IgG from a 0.1 mg/mL solution, using identical EDV/S-NHS immobilization conditions in concordance with each manufacturer's protocols. Anti-human IgG was applied at 0, 100 ng/mL, 1 μg/mL, 10 μg/mL, and 100 μg/mL in ascending order. Each chip was regenerated by incubation with 25 mM glycine buffer, pH 2.0 for 20 minutes between exposures. Kinetic traces were displayed with SKi Pro A) or Biacore 3000's proprietary software B). Concentration response curves were drawn in OPD (SKi Pro) and RU (Biacore) agains the concentration in μg/mL. Limit of detection for this particular protein pair is 100 ng/mL on the SKi Pro, and 1 μg/mL on the Biacore 3000 platform C).