Figure 2

2D-gel analysis of Histoplasma capsulatum proteins following A) 3-day growth in liquid culture. B) 2-day growth in liquid culture followed by 24 hr with growth media containing 5 μM apo-transferrin. Protein spot circled in black is apo-transferrin C) 4-day growth in liquid culture D) 2-day growth followed by 48 hr with growth media containing 5 μM apo-transferrin. Proteins selected as statistically different in their protein abundance between control and apo-transferrin treated are circled and numbered in white by the SameSpots sofware. The pH gradient is labeled on the top of the gel and molecular weight markers are labeled in Daltons on the right. Gels were loaded with 50 μg of total protein and stained with SYPRO Ruby fluorescent dye.