Figure 4
From: Histoplasma capsulatum proteome response to decreased iron availability
Western blot analysis of proteins extracted from H. capsulatum grown with apo-tranferrin for 48 hr versus cultures free of apo-transferrin. A) using antibody against Cyclophilin (S41, Figure 1) which showed no statistically different changes in protein abundance from our 2D gel analysis. B) using antibody against HSP70 which was found more abundant following 2D gel analysis in the medium free of apo-transferrin compared to medium containing apo-transferrin. C) Graphical representation of spot density calculation from biological replicates probing for HSP 70, p value = .034.