Table 1 Overview on instrumentation and consumables
From: Automated production of recombinant human proteins as resource for proteome research
| Â | Robot | Periphery | Consumables |
|---|---|---|---|
Gateway cloning | MultiProbe II EX, PerkinElmer, Wellesley | VortexGenie 2, Scientific Industries, Bohemia GeneAmp PCR System 9700, Applied Biosystems | Thermo-Fast 96 (ABgene, Epsom) |
Transformation | MultiProbe II EX, PerkinElmer, Wellesley | GeneAmp PCR System 9700, Applied Biosystems | Thermo-Fast 96 (ABgene, Epsom) QTray, Vented (Genetix, New Milton) |
Clone Picking | QPix (Genetix, New Milton) | Â | 6.0 mL Storage Plate with 48 Wells (ABgene) |
Plasmid purification | MultiProbe II EX, PerkinElmer, Wellesley | Teleshake (H+P Labortechnik, München) Vacuum Manifold for MultiScreen Plates (Millipore) | 2.2 mL Storage Plate (ABgene) Montage Plasmid Miniprep96 Kit (Millipore) |
Restriction digest | MultiProbe II EX, PerkinElmer, Wellesley | Â | Thermo-Fast 96 (ABgene, Epsom) |
Affinity purification | MultiProbe II EX, PerkinElmer, Wellesley |  | ProtinoTrap M96/20 μm/L (M&N, Düren) |