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Table 4 Buffers and materials used for protein purification

From: Automated production of recombinant human proteins as resource for proteome research

  His-Tag GST-Tag MBP-Tag
Resin Ni-NTA Superflow (QIAGEN) Glutathione Sepharose 4B (Amersham Biosciences) Amylose resin (New England Biolabs)
Resuspension/extraction 50 mM NaH2PO4, pH 8 300 mM NaCl 10 mM imidazole adjust pH w/NaOH 20 mM Tris-HCl, pH 8 500 mM NaCl 1 mM EDTA 1 mM DTT 20 mM Tris-HCl, pH 7.4 200 mM NaCl 1 mM EDTA
Wash 50 mM NaH2PO4, pH 8 300 mM NaCl 20 mM imidazole adjust pH w/NaOH PBS, pH 7.3 20 mM Tris-HCl, pH 7.4 200 mM NaCl 1 mM EDTA
Elution 50 mM NaH2PO4, pH 8 300 mM NaCl 250 mM imidazole adjust pH w/NaOH PBS 20 mM glutathione (red.) Wash buffer 10 mM maltose