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Table 1 Mycobacterium avium subsp paratuberculosis proteins used in this study

From: Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle

Protein namea

Concentration (mg/ml)b

Gene sizec

Amino acidsd

Predicted function

MAP sonicate

1.0

NAe

NA

NA

MAP0075

0.18

423

140

conserved small membrane protein

MAP0087-his

1.0

717

238

probable secreted protein

MAP0105c

1.24

1058

352/889

hypothetical protein

MAP0182c

0.67

411

136

conserved hypothetical protein

MAP0216

1.66

1028

342/347

antigen 85A, mycolyltransferase

MAP0261c

0.49

465

154/161

19 kDa protein

MAP0389

2.04

947

315/337

diarylpropane peroxidase (EC 1.11.1.14) [Nostoc]

MAP0736

5.73

744

247

hypothetical protein

MAP0852

10.4

546

181

no BLAST hits

MAP0853

4.04

660

219

no BLAST hits

MAP0855

0.34

926

308/314

no BLAST hits

MAP0857c

0.70

318

105

hypothetical protein

MAP0858

0.44

537

178/182

no BLAST hits

MAP0859c

0.66

609

202

Mycobacterium phage L5

MAP0860c

0.04

885

294/296

no BLAST hits

MAP0861

0.43

342

113

no BLAST hits

MAP0862

0.48

989

329/360

no BLAST hits

MAP0863

0.66

675

224

no BLAST hits

MAP0864

7.42

426

141

no BLAST hits

MAP0865

0.08

1272

423

Hypothetical 30.9 kDa protein

MAP0866

3.07

804

267

integrase

MAP0904

0.81

371

124/246

conserved hypothetical protein

MAP0961c

0.18

719

239/352

hypothetical protein

MAP1087

0.43

441

146

prob. peptide transp. system permease, N-terminal

MAP1121c

0.32

498

165

putative substrate binding protein

MAP1174c

1.64

756

251

glucose-6-phosphate 1-dehydrogenase

MAP1204

2.61

735

244

putative exported p60 protein homologue

MAP1233

0.04

723

240

conserved hypothetical protein

MAP1345

0.69

600

199

no BLAST hits

MAP1388

0.08

396

131

no BLAST hits

MAP1416c

0.45

666

221

no BLAST hits

MAP1417c

0.54

435

144

no BLAST hits

MAP1609c

0.05

987

328/330

antigen 85B, mycolyltransferase

MAP1636c

1.12

425

141/157

no BLAST hits

MAP1643

0.45

2277

759/762

isocitrate lyase, [beta] module

MAP1655c

0.93

381

126

hypothetical protein

MAP1730c

0.26

1023

340

putative ATP/GTP-binding protein

MAP1931c

0.32

1104

367

anthranilate phosphoribosyltransferase

MAP2077c

1.66

330

109

hypothetical protein

MAP2116c

0.27

1269

422

cell invasion protein

MAP2121c

7.14

887

295/307

major membrane protein I

MAP2121c-his

0.99

924

307

major membrane protein I

MAP2151

2.79

438

145

no BLAST hits

MAP2155

9.57

312

103

similar to IS6110

MAP2156

0.59

963

320

putative transposase GEN: X52471)

MAP2157

0.38

1221

406

IS900

MAP2158

2.40

582

193

no BLAST hits

MAP2182c

0.26

435

144

conserved hypothetical protein

MAP2231

0.31

1422

473

PKS-associated protein, unknown function

MAP2360c

0.13

534

177

hypothetical protein

MAP2380

2.00

600

200/550

acyl-CoA synthase

MAP2657

0.26

651

216

putative oxidoreductase

MAP2663c

0.03

510

169

putative integral membrane protein

MAP2676c

1.30

354

117

hypothetical protein

MAP2734

0.37

1341

446

putative dioxygenasesdiooxygenases

MAP2740

0.24

666

221

putative membrane protein

MAP2751

0.70

582

193

no BLAST hits

MAP2753

0.17

309

103/252

hypothetical protein

MAP2761c

0.03

692

230/238

no BLAST hits

MAP2762c

1.45

425

141/146

no BLAST hits

MAP2764c

0.10

290

96/149

no BLAST hits

MAP2765c

0.06

539

179/396

no BLAST hits

MAP2767c

0.42

473

157/183

no BLAST hits

MAP2963c

0.88

1499

499/874

no BLAST hits

MAP3084c-his

1.0

684

227

probable secreted protein

MAP3121

0.38

855

284

enoyl-CoA hydratase/isomerase superfamily

MAP3129

0.09

404

134/141

hypothetical protein

MAP3131

0.30

1096

365/942

conserved large membrane protein

MAP3155c

1.22

273

90

hypothetical protein

MAP3434

0.18

690

230/330

possible membrane protein

MAP3437c

0.29

843

279/280

no BLAST hits

MAP3531c

0.28

1034

344/352

antigen 85C, mycolytransferase

MAP3734c

0.19

1559

519/593

putative ABC transporter ATP-binding protein

MAP3735c

0.42

1181

393/429

part of heavy metal tolerance protein

MAP3743

0.85

998

332/348

hypothetical protein

MAP3751

0.11

879

293/979

conserved large membrane protein

MAP3753

0.64

1353

450

hypothetical protein

MAP3761c

0.07

729

242

conserved membrane protein

MAP3771

0.02

294

97

50S ribosomal protein L31

MAP3817c

0.65

939

312

no BLAST hits

MAP3833c

0.13

626

208/260

hypothetical protein

MAP3840

0.10

1872

623

70 kD heat shock protein, chromosome replication

MAP3902c

0.15

522

173

Serine/threonine kinase (EC 2.7.1.37) [Myc...

MAP3903c

0.42

531

176

Serine/threonine kinase (EC 2.7.1.37) [Myc...

MAP3954

0.05

279

92

conserved hypothetical protein

MAP4014

0.21

951

316

probable 4-amino butyrate transporter, N-terminal

MAP4025

0.32

854

284/325

cytochrome c-type biogenesis protein

MAP4129

0.26

965

321/336

ABC transporter

MAP4198

0.13

1283

427/441

SecY subunit of preprotein translocase

MAP4207c

5.28

686

228/231

probable ATP-binding transport protein

MAP4199

0.03

540/546

179/181

probable adenylate kinase

MAP4228

0.05

222

73

initiation factor IF-1

  1. aProtein name corresponds to the genome project designation (Li et al., 2005). A second designation is included if known.
  2. bProtein concentration is reported in as measured by Nanodrop spectrometry at 280nm.
  3. cGene size is reported in base pairs (bp). In cases where only a portion of the M. avium subsp paratuberculosis gene was cloned, the number of bp present in the clone is reported first followed by the number of bp in the full-length M. avium subsp paratuberculosis gene.
  4. dNumber of M. avium subsp paratuberculosis amino acids present in the fusion protein. In cases where only a portion of the M. avium subsp paratuberculosis protein is represented, the number of amino acids present is listed first followed by the total number of M. avium subsp paratuberculosis amino acids in the full-length protein.
  5. eNA is not applicable.