Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle

Bannantine, John P; Bayles, Darrell O; Waters, W Ray; Palmer, Mitchell V; Stabel, Judith R; Paustian, Michael L

doi:10.1186/1477-5956-6-5

Proteome Science

Table 2 Spot intensities of proteins present on the array.^a

From: Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle

		Cow 5902				Cow 5904
Spot Loc^b	Protein	Day 0	Day 70	Day 194	Day 321	Day 0	Day 70	Day 194	Day 321
A7	MAP0075	0	4.472	21.431	21.122	0	0	27.612	21.36
A12	MAP0087-his	0.978	0	0	0	1.112	1.882	0	1.228
A5	MAP0105c	0	6.929	3.867	5.096	1.186	2.321	10.106	1.813
B5	MAP0182c	0	5.407	0	1.924	1.177	2.409	1.617	22.366
C4	MAP0216	0.652	3.779	1.296	1.395	2.175	3.444	0	0
G4	MAP0261c	2.395	2.287	3.487	5.668	1.097	3.146	2.397	2.496
E8	MAP0389	0	7.809	3.163	1.817	0	1.533	16.751	5.529
F8	MAP0736	0.734	0	0	0	0	0	1.446	0
A1	MAP0852	2.257	3.747	2.908	1.147	2.564	4.661	2.709	1.537
B1	MAP0853	2.144	3.734	5.095	8.497	1.961	4.158	3.239	2.649
C1	MAP0855	2.538	3.387	4.552	7.898	1.716	2.248	2.132	0
D1	MAP0857c	2.709	6.445	21.867	37.718	1.045	1.602	23.425	15.825
E1	MAP0858	3.005	3.882	1.775	3.999	1.978	0	0	0
F1	MAP0859c	2.821	4.137	2.87	3.745	1.909	0	2.803	0
G1	MAP0860c	2.996	0	5.531	12.743	1.592	0	7.669	6.676
H1	MAP0861	5.074 ^c	0	3.407	3.053	1.546	4.05	3.111	3.52
A2	MAP0862	1.795	40.036	16.196	7.997	2.796	11.451	30.918	12.422
B2	MAP0863	1.518	5.524	3.174	4.592	2.057	4.891	3.274	1.473
C2	MAP0864	1.255	3.44	1.781	1.726	2.016	3.56	0	0
D2	MAP0865	1.909	18.708	32.138	44.846	1.317	4.386	31.361	35.251
E2	MAP0866	2.346	7.051	2.67	7.116	1.315	3.561	4.052	6.009
G8	MAP0904	1.678	0	0	0	0	0	0	0
G5	MAP0961c	1.797	14.366	26.5	37.546	0.78	2.103	33.046	42.877
A11	MAP1087	0.753	53.179	51.634	70.968	0.874	33.469	75.392	68.964
B11	MAP1121c	0.758	2.438	0	2.094	0.699	3.364	0	1.848
A10	MAP1174c	0	2.301	3.155	4.411	0.623	1.921	5.81	4.955
C11	MAP1204	0.78	40.8	43.856	55.046	0	28.367	61.122	58.985
F2	MAP1233	2.568	12.375	22.507	32.493	1.932	3.212	24.157	30.447
C5	MAP1345	0	3.339	1.861	1.018	1.147	0	3.413	2.389
D5	MAP1388	0	2.281	0	0	0.429	2.835	0	0
E5	MAP1416c	0	1.647	0	0	0.791	3.791	0	0
F5	MAP1417c	1.052	14.882	4.095	6.212	0.955	3.979	7.614	3.521
D4	MAP1609c	0.733	3.916	0	0	1.003	5.292	0	0
G2	MAP1636c	3.286	6.944	13.249	17.272	1.701	0	17.226	12.06
B4	MAP1643	0	4.155	0	1.534	1.827	0	0	0
H8	MAP1655c	2.788	0	0	0	0	2.049	0	0
G6	MAP1730c	1.147	29.831	43.872	52.35	0	12.272	57.799	57.47
B10	MAP1931c	0	2.917	0	2.217	0.511	2.886	2.077	3.339
H5	MAP2077c	3.833	0	2.784	9.443	1.385	0	3.63	9.221
D11	MAP2116c	1.418	29.88	33.215	42.633	0.568	11.24	51.336	47.918
F4	MAP2121c	1.76	4.158	1.373	1.513	0.969	3.005	0	0
B12	MAP2121c-his	1.247	0	0	0	0.889	2.172	21.226	2.155
H2	MAP2151	4.136	1.919	12.688	21.897	1.571	3.402	24.619	20.889
F12	MAP2155	2.434	0	1.385	0	1.251	2.039	0	2.105
G12	MAP2156	3.731	2.327	0	2.12	1.357	2.647	5.42	5.113
H6	MAP2157	3.504	0	0	0	0.751	1.532	1.51	2.683
A3	MAP2158	1.403	3.487	4.2	3.396	3.187	1.704	6.682	2.204
A9	MAP2182c	0	19.101	7.349	8.933	0	0	27.935	12.096
E11	MAP2231	1.846	3.732	11.54	15.671	1.166	0	17.203	18.933
B9	MAP2360c	0	3.168	0	0	0	2.766	0	0
C10	MAP2380	0.772	2.139	1.198	0	0	0	1.968	3.225
D10	MAP2657	0	22.372	8.29	3.68	0	15.937	39.178	25.032
B7	MAP2663c	0	9.594	3.661	2.248	0.868	0	4.9	2.717
A6	MAP2676c	0	3.291	1.406	0	0	0	2.036	0
D7	MAP2734	2.726	9.109	11.031	9.269	0	0	27.935	18.79
C7	MAP2740	0	1.736	6.447	18.061	0.507	0	6.295	10.804
B6	MAP2751	0	3.157	1.101	2.373	0.8	0	1.729	2.206
C6	MAP2753	0	1.909	0	2.176	0.624	0	1.701	2.585
D6	MAP2761c	0	0	0	0	0	0	0	0
E6	MAP2762c	0	0	0	0	0	2.397	0	0
B3	MAP2764c	1.472	2.739	1.969	5.874	2.13	5.442	2.039	1.955
H11	MAP2765c	4.816	0	0	0	0	4.799	0	0
C3	MAP2767c	1.257	3.507	2.303	7.019	1.982	4.347	1.92	2.722
F6	MAP2963c	0	0	0	0	0	2.204	0	0
C12	MAP3084c-his	1.313	0	0	0	0.999	2.026	0	1.982
E10	MAP3121	1.079	0	0	0	0	0	0	0
C9	MAP3129	1.406	2.366	0	1.206	0	0	1.731	2.865
E7	MAP3131	0	0	0	0	0	0	1.404	1.713
G11	MAP3155c	2.783	2.553	2.725	3.841	1.435	2.237	2.226	4.865
F7	MAP3434	0.832	1.401	8.312	19.522	0.508	0	33.289	14.418
D3	MAP3437c	1.674	3.321	1.29	1.587	1.359	1.965	0	0
E4	MAP3531c	0.926	7.373	2.472	4.313	0.558	2.834	1.374	0
G7	MAP3734c	1.085	2.238	2.719	17.809	0	0	10.914	20.81
E3	MAP3735c	1.944	4.897	5.078	18.618	1.326	3.368	3.887	4.502
F3	MAP3743	2.113	23.874	16.282	16.773	1.28	6.953	21.309	22.067
H7	MAP3751	2.991	0	0	0	0	0	2.499	3.033
D9	MAP3753	0	2.912	7.043	3.212	0	0	18.95	15.247
A8	MAP3761c	0	2.473	14.931	23.178	0	0	18.02	21.264
G3	MAP3771	2.81	2.229	1.344	2.528	0.953	1.997	0	0
H3	MAP3817c	3.836	2.526	10.298	22.826	1.101	1.552	15.736	19.901
E9	MAP3833c	1.078	0	2.07	2.755	0	0	3.676	4.636
A4	MAP3840	0.823	4.968	3.842	4.521	1.403	0	10.083	0
F9	MAP3902c	1.095	0	0	0	0	2.376	0	0
G9	MAP3903c	1.755	1.355	0	1.027	0	1.772	0	0
H9	MAP3954	3.426	0	0	1.969	0	7.584	1.612	3.629
F11	MAP4014	2.266	2.035	5.415	8.98	0.545	0	5.293	8.043
F10	MAP4025	1.474	25.139	34.876	39.022	0	13.413	49.655	47.012
B8	MAP4129	0	1.489	0	1.363	0	0	1.435	2.585
C8	MAP4198	0	1.536	1.517	6.817	0	0	5.431	6.175
G10	MAP4199	1.919	1.688	0	0	0.821	2.339	0	0
D8	MAP4207c	1.981	1.691	1.872	1.364	0	0	3.699	5.632
H10	MAP4228	4.373	0	0	0	0	3.122	0	0
H4	MBP-LacZ	4.128	0	0	1.722	1.303	0	0	0
D12	PBS buffer^d	1.676	0	1.236	0	0.857	2.322	0	2.003
E12	Sonicate^e	2.673	0	0	0	1.287	1.936	2.963	3.523
H12	Sonicate	5.01	1.359	0	0	1.366	4.337	3.002	4.472

^aListed are the intensity values for each spot arranged by ascending MAP number. Measurements were made using a consistent 1804 pixel area circle to record spot density using the Adobe Photoshop CS3 Extended application.
^bLoc is location on the dot blot array.
^cThe strongest intensity for each time point is shown in bold.
^dPBS buffer in spot D12 represents the spotting buffer only control.
^eSonicate is a sonicated whole cell lysate of M. avium subsp paratuberculosis.

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ISSN: 1477-5956

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