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Table 3 Culture and immunological status of cattle used in this study.

From: Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle

   

IFN-γ (mg/ml)c

 

Cow ID

Survey datea

Fecal culture (CFU)b

NS

ConA

MPS

ELISA (S/P ratio)d

85

12/20/05

C, C, C, C

0.069

0.454

0.278

0.002

 

3/4/06

2, 2, 2

0.061

0.185

0.068

ND

112

12/20/05

C, C, 21, 1

0.07

0.218

0.064

0.027

 

4/10/06

2, 1, 1

0.063

1.83

0.09

0.014

113

8/10/04

C, C, C, C

0.21

0.514

0.054

0.041

 

11/10/04

0, 3, 1

0.39

1.51

0.54

0.09

117

12/17/04

T, T, T, T

0.053

0.143

0.097

-0.052

 

3/1/05

T, C, C

0.039

0.174

0.046

ND

183

12/20/05

0, 0, C, C

0.054

0.241

0.145

1.42

 

4/7/06

3, 2, 0

0.089

1.134

0.194

0.981

235

12/20/05

0, 0, 0, 0

0.068

0.362

0.114

0.039

 

3/4/06

0, 1, 1

0.05

0.35

0.064

0.021

834

8/10/04

0, 1, 0, 1

0.033

0.336

0.142

0.87

 

11/11/04

0, 0, 0

0.044

0.806

0.076

0.648

871

8/10/04

C, C, C, C

0.037

0.361

0.054

0.049

 

12/21/04

C, 2, 1

0.043

0.189

0.064

0.121

2715

12/20/05

2, 0, 1, 0

0.067

1.3

0.324

0.226

 

3/18/06

0, 0, 0

0.059

0.211

0.062

0.044

  1. aEach animal is surveyed quarterly to monitor progression of Johne's disease. The two most recent quarterly surveys are shown for each animal, but only sera collected on the most recent date was used for the immunoblot assay in Figure 4.
  2. bFecal culture is reported as numbers of colony forming units (CFU) present on each HYEM slant (platings are in triplicate or quadruplicate). A "C" indicates the slant had bacterial growth other than M. paratuberculosis. A "T" designation means too many colonies to count on that slant.
  3. cThe gamma interferon (IFN-γ) assay. Subclinical cows typically have a M. paratuberculosis sonicate (MPS) reading higher than the no stimulation (NS) reading, along with little or no colonies on fecal culture slants. The concanavalin A (ConA) reading is a positive control as cells are readily stimulated by this lectin protein.
  4. dThe ELISA sample to positive (S/P) ratio is calculated from the optical density reading as described previously [11].
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