Proteomic profiling of endorepellin angiostatic activity on human endothelial cells

Table 1 Differential expression of endothelial cell proteins evoked by exposure to recombinant human endorepellin

*Spot number (see Fig. 2)*	*Protein ID*	*Gene name*	*NCBI Accession Number*	*Mr (kDa)*	pI	*Response to endorepellin (mean fold change)*	*p-value*	*Reported functions* ^a
1	Calreticulin	CALR	4757900	48	4.14	↓ Downregulated, 0.642	0.0881	Molecular calcium binding and chaperone protein
2	β-actin	ACTB	4501885	42	5.18	↓ Downregulated, 0.403	0.0023	Cellular structure, motility and integrity
3	Chaperonin/Hsp60	HSPD1	31542947	61	5.59	↓ Downregulated, 0.714	0.1262	Mitochondrial protein assembly, prevention of misfolding, produced under stress within the mitochondrial matrix
4	Vimentin	VIM	4507895	54	4.91	↑ Upregulated, 3.178	0.0276	Class III intermediate filament, cytoskeletal crosstalk, intracellular communication, influence cell shape, adhesion and migration, signalling events
5	Prolyl 4-hydroxylase, β subunit	P4HB	20070125	57	4.61	↑ Absent from Control^b	-	Key subunit of prolyl 4-hydroxylase enzyme, identical to protein disulfide isomerase

^aAs reported in the protein database on the ExPASy web server and in the NCBI Entrez Protein database.
^bTrace amounts of this enzyme were found by immunoblotting (see Figure 3 E)

ISSN: 1477-5956