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Table 3 The expression pattern of genes as analyzed by Quantitative real time RT-PCR

From: A study on the differential protein profiles in liver cells of heat stress rats with and without turpentine treatment

Gene

HS group

T+HS group

 

Mean fold changes (2 -ΔΔCT ) c

SD a

CV b

Mean fold changes (2 -ΔΔCT ) c

SD

CV

FAH

-4.55*

0.76

16.76

-11.18*

1.27

11.34

FBP1

-1.06

0.05

4.72

-1.23

0.15

12.14

ASS

-2.21*

0.33

15.12

-2.07*

0.41

19.74

DMGDH

2.73*

0.57

20.95

-2.99*

0.69

23.31

PK

10.12*

0.81

8.02

19.11*

2.18

11.4

TKT

4.67*

0.66

14.13

-1.30

0.30

22.96

PPIA

18.01*

2.21

12.27

3.02*

0.64

21.06

CEH

34.54*

7.92

22.94

4.69*

0.65

13.90

  1. The fold changes are mean of three values compared to control groups and using a cut-off of ≥ ± 2.0 for significance (*P < 0.05).
  2. (a) The standard deviation (SD) quantifies variability or scatter of a data set, and is expressed in the same units as the data. If the data are sampled from a Gaussian distribution, then 95% of the values is expected to lie within two SD of the mean.
  3. (b) Coefficient of variation (CV) represents the ratio of the SD to the mean of the data and indicates the relative variability of SD value to the mean value of the datasets.
  4. (c) The samples were analyzed using QRT-PCR and the Ct data were imported into Microsoft excel. The mean fold change in expression of the target gene at HS and T+HS was normalized relative to the house keeping gene β-actin and calculated by the formula 2-ΔΔCT, where ΔΔ CT = (CT Target – CT Actin) treated – (CT Target – CT Actin) control. The mean ± SD values of β-actin calculated for control, HS and T+HS were 18.72 ± 0.516, 24.04 ± 0.106 and 25.78 ± 0.092 respectively.