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Table 1 2D-DIGE experimental design

From: Two-dimensional gel proteome reference map of human small intestine

Gel number Cy2 Cy3 Cy5
1 Pooled standard Sample 1 Sample 2
2 Pooled standard Sample 3 Sample 4
3 Pooled standard Sample 5 -
  1. For DIGE minimal labelling, 25 μ g of protein sample were mixed with 100 pmol CyDye (GE Healthcare). Every gel included 2 different samples, labelled with a different CyDye, and a Cy2-labelled internal standard, which is a pool of all the samples within the experiment.