Figure 2
From: Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803
Separation of membrane associated proteins by 2-DE. The membrane associated proteins in the peripheral fraction were separated using 18-cm IPG strips covering pH ranges 3–10 (nonlinear), 4–7, 4–5, and 5–6 for the first dimension, and then separated by 12–18% gradient SDS-PAGE gels for the second dimension. Each gel shown is a representative of the three replicates that has the best resolution. All the labeled protein spots in the 2-D gels were analyzed by mass spectrometry and identified using peptide mass fingerprinting.