Figure 3
From: Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803
Separation of integral membrane proteins by 2-DE. The integral proteins were separated using 18-cm IPG strips covering pH ranges 3–10 (nonlinear), and 4–7 for the first dimension, and then separated by 12–18% gradient SDS-PAGE gel in the second dimension. Each gel shown is a representative of the three replicates that has the best resolution. All the labeled protein spots in the 2-D gel were analyzed by mass spectrometry and identified using peptide mass fingerprinting.