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Figure 3 | Proteome Science

Figure 3

From: Proteomic analysis of phosphoproteins sensitive to a phosphatidylinositol 3-kinase inhibitor, ZSTK474, by using SELDI-TOF MS

Figure 3

Inhibitory effects of ZSTK474 on phosphoproteins. Phosphorylation of Akt (Ser473), p70S6K (Thr389), 4E-BP1 (Ser65, Thr70, Thr37/46) and total 4E-BP1 were measured in 4E-BP1 over-expressing A549 cells by immunoblotting with respective antibodies. The cells were treated with indicated concentrations of ZSTK474 (0.25, 0.5, 1, 2, and 4 μM) or rapamycin (0.1, 0.2, 0.4, 0.8, and 1.6 μM) for 30 min. Intensity ratio of phosphorylated form/total form of 4E-BP1 was indicated under each lane. ZSTK474 inhibited phosphorylations of each downstream component of PI3K in a dose dependent manner. In contrast, rapamycin did not inhibit phosphorylation of Thr37/46 in 4E-BP1 as well as that of Ser473 in Akt.

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