Comprehensive proteomic analysis of human cervical-vaginal fluid using colposcopy samples

Table 3 Overview of studies performed on human CVF.

*Study*	*Samples*	*Part of sample analyzed*	*Separation method*	*MS method*
Venkataraman et al., 2005[29]	Undiluted CVF collected in cup from healthy women (postmenarcheal, pre-menopausal)	Cationic fraction	2D-PAGE (1D: AU-PAGE; 2D: Tricine- SDS-PAGE)	MALDI-TOF-TOF
Di Quinzio et al., 2007[26]	Swabs from pregnant women (37 weeks gestation)	Only protein spots identified common to five gels	2D-PAGE (1^stD: IEF; 2^ndD SDS-PAGE) followed by RP-LC	MALDI-TOF or ESI-linear IT
Dasari et al., 2007[24]	Swabs from pregnant women (18,5 weeks gestation as mean)	Whole CVF	1D-SDS-PAGE followed by offline 2D(SCX/RP)-LC	ESI-Q-TOF
Tang et al., 2007[11]	Washings from clinically normal women; 7 washings from women infected with Candida spp.	Whole CVF	2D-PAGE (1^stD: IEF; 2^ndD SDS-PAGE)	MALDI-TOF-TOF
Shaw et al., 2007[28]	Gauze from healthy women	Whole CVF	1D-SDS-PAGE or SCX-LC both followed by RP-LC	ESI-linear IT
Pereira et al., 2007[27]	Swabs from pregnant women (15.8–35.9 weeks gestation)	Whole CVF	2D-DIGE or MudPIT(SCX/RP)-LC	ESI-Q-TOF
Klein et al., 2008[25]	Swabs from pregnant women (30.5 weeks gestation as mean)	Whole CVF	RP-LC	ESI-IT
This study	Washings from HPV-infected women	Whole CVF	Ultrafiltration or C₄-LC protein fractionation/C₁₈-LC peptide separation	MALDI-TOF-TOF

For each study the following information is presented: 1) the nature of the samples which were used in the study (sample collection method and patient physiology), 2) whether the study focused on a particular part of the sample or analyzed the whole CVF, 3) the methods used to separate proteins and peptides and 4) MS method used for analysis.

ISSN: 1477-5956