Figure 3

Profiles of proteins belonging to six categories of mitochondrial pathways. The pathways are: A) Citric acid cycle together with pyruvate dehydrogenase (PDH), B) Respiratory chain, C) Fatty acid oxidation, D) Antioxidant systems, E) Protein quality control and F) Apoptosis and mitochondrial morphology. The galactose to glucose ratio, derived from the protein levels of the cells cultivated in galactose and glucose, respectively, is depicted on the x-axis. Black bars indicate ratios calculated from the average of three independent cultivation studies of a control fibroblast (NHDF-1). The grey bar indicates ratio from one cultivation study of a second control fibroblast (NHDF-2). The ratio of a protein was reported as significantly different from 1.0 if it passed two tests 1) a threshold test of two times the global standard error (2 × 0.055 = 0.11) and 2) a two-tailed student's T-test for equal variance data. The error bar is the standard error of three values and "*" and "**" indicate statistically significant deviation from 1.0 with a t-test probability value below 0.05 and 0.01, respectively. A few proteins have activity in two pathways and are then depicted two times. The proteins in pathway A, B and C were sorted according to their position in the pathway, whereas the proteins in pathway D, E and F were alphabetically sorted based on the HGNC symbol, i.e. the letters that precedes the protein description.