Altered proteolytic events in experimental autoimmune encephalomyelitis discovered by iTRAQ shotgun proteomics analysis of spinal cord

Table 1 Altered non-tryptic cleaved peptides in EAE rat spinal cord

Protein	Swiss-Prot Accession Number	Peptide ^a	Observed Mass (m/z)	Error (ppm)	Peptide Ratio^b ± SD	p-Value	Protein Ratio (N) ^c	Sequence Coverage (%)
α ₁B-glycoprotein	Q9EPH1\|A1BG	GPGNA-²¹LWLDSGSEPELR³²-AEPQS	1545.743	38	4.3 ± 1.2	0.02	3.3 (5)	6
α₁-Macroglobulin	Q63041\|A1M	VFQPF-⁸⁰⁷FLELTLPYSVVR⁸¹⁸-GEAFI	1580.917	0	5.9 ± 1.6	0.03	2.8 (19)	13
β₂-Microglobulin	P07151\|B2MG	DWSFY-⁸³ILAHTEFTPTETDVYACR¹⁰¹-VKHVT	2257.067	4	3.1 ± 0.8	0.02	2.7 (2)	26
Neurofilament light polypeptide	P19527\|NFL	AFPAY-⁴⁴³YTSHVQEEQSEVEETIEATK⁴⁶³-AEEAK	2625.292	6	2.2 ± 0.6	0.05	0.9 (75)	56
Phospho glucomutase-1	P38652\|PGM1	KFKPF-¹⁸⁴TVEIVDSVEAYATMLR²⁰⁰-NIFDF	1941.006	-3	1.6 ± 0.1	0.01	1.1 (6)	17
Sulfated glycoprotein 1	P10960\|SAP	QPKAN-¹⁹⁴EDVCQDCMK²⁰²-LVTDI	1450.59	11	2.4 ± 0.2	0.00	1.9 (16)	19
Sulfated glycoprotein 1	P10960\|SAP	PQKNG-⁴³⁷GFCEVCK⁴⁴³-KLVIY	1165.508	-3	4.5 ± 0.7	0.00	1.9 (16)	19

^a Sequences bracketed by amino acid numbers are the semi-tryptic peptides discovered experimentally. Five amino acids before and after the semi-tryptic peptides are shown to indicate the non tryptic cleavage sites.
^b iTRAQ Ratio: EAE/Control
^c Total number of peptides identified for respective protein (see Additional file 2)

ISSN: 1477-5956