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Figure 3 | Proteome Science

Figure 3

From: Proteomic profiling of glucocorticoid-exposed myogenic cells: Time series assessment of protein translocation and transcription of inactive mRNAs

Figure 3

RPS13 increases in the cytosol in response to prednisone. Quantitation of the zoom scans for three unique peptides identified in the cytosolic fraction for RPS13. The mean ratio for RPS13 was multiplied by each timepoint's normalization factor (NF), which was calculated to adjust the overall protein population mean ratio to 1.0. Peptides were normalized to their corresponding baseline peptide at T0, resulting in a normalized peptide ratio (NR). The NR was then converted to a fold change (FC). Results showed an increase in RPS13 levels detectable through T15 (15 minutes) post-exposure. Quantitation was performed on the intercalating zoom scans for the corresponding MS2 scan by taking the ratio of the monoisotopic peak of the heavy (labeled) peak divided by the monoisotopic peak of the light (unlabeled, prednisone-exposed) peak.

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