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Table 1 Proteins whose expression is consistently increased after 8 hour treatment with IGF-1.

From: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts

Spot Number Protein Name NCBI gi # Fold Increase SC (%) Z-score MW; pI (theoretical) MW; pI (actual)
  Adaptor Protein:       
1 CASK interacting protein 1 161168996 3.8 ± 0.2 11 2.29 141.42/9.5 120/8.5
2 Wbscr1 protein 15928652 3.9 ± 0.4 41 1.93 25.22/7.8 27/7.5
  Cell surface receptor:       
3 Stabilin 1 26352640 4.0 ± 0.4 17 2.09 49.21/8.0 55/8.0
  Cytoskeletal/Structural:       
4 GRIP and coiled-coil domain-containing protein 2 32469711 3.2 ± 0.1 12 1.75 195.40/5.1 170/5.8
5 Rho interacting protein 3 56205310 3.3 ± 0.1 12 1.27 115.70/6.0 140/6.5
6 Vimentin 55408 5.3 ± 0.6 23 1.93 53.72/5.1 55/5.6
  DNA Binding protein:       
7 RAD50 1575575 5.0 ± 0.4 14 1.59 154.61/6.5 80/7.8
  Enzyme: Hydratase       
8 Enolase (phosphopyruvate hydratase) (EC 4.2.1.11) alpha 109803 4.7 ± 0.3 29 1.90 47.45/6.4 50/5.7
  Enzyme: Isomerase       
9 Triose-phosphate isomerase 1363194 3.8 ± 0.1 27 2.01 27.02/6.9 30/7.5
  Enzyme: Sulphotransferase:       
10 N-deacetylase/N-sulfotransferase 4 11344505 3.7 ± 0.3 10 1.74 101.24/6.9 100/6.5
  Extracellular matrix:       
11 Collagen alpha-1(XVII) chain 41688459 3.5 ± 0.4 8 1.38 148.48/9.0 160/7.2
  Heat shock protein (Chaperone):       
12 Heat shock protein 8 42542422 3.3 ± 0.2 21 1.39 71.08/5.3 78/5.3
13 Heat shock protein A/p66 mot 34784211/5736598 4.1 ± 0.4 14   73.80/5.9 62/5.2
  Phosphotransferase:       
14 FLT-1 (Tyrosine-protein kinase receptor FLT) 2809069 3.1 ± 0.2 8 1.53 151.71/9.1 160/10
15 Ikbkb protein 22902306 3.6 ± 0.4 22 1.60 87.91/5.7 80/7.8
  Reductase:       
16 pyrroline-5-carboxylate reductase-like 119508439 4.1 ± 0.4 27 1.32 29.13/6.8 36/7.9
  Ribonuclease:       
17 Plenty-of-prolines-101; POP101; SH3-philo-protein 3153821 5.6 ± 1.0 20 1.68 101.21/11.9 110/6.2
  Transcription factor:       
18 NFKB-repressing factor-Transcription factor NRF 12861044 3.0 ± 0.1 13 1.49 77.71/9.1 33/4.5
  Unclassified/Unknown function:       
19 Cep290 26353954 4.1 ± 0.3 33 2.08 93.89/7.6 95/7.6
20 mKIAA0209 protein/dedicator of cytokinesis 2 50510415 3.2 ± 0.1 16 1.45 182.35/6.7 125/7.5
21 mKIAA1486 protein 81896693 3.0 ± 0.3 13 1.95 71.19/9.1 63/8.5
22 nasopharyngeal epithelium specific protein 1   3.4 ± 0.1 30 2.08 65.98/9.0 75/9.0
23 progesterone-induced blocking factor 1 isoform a 46852193 3.3 ± .03 21 1.73 89.88/5.9 90/6.8
  1. Note 1: Protein classification is according to the Human Protein Reference Database http://www.hprd.org.
  2. Note 2: SC%: Percentage of sequence coverage.
  3. Note 3: Spot Number refers to the location of the individual proteins on 2D gels as shown in Figures 3–6. When a protein was identified in more than one Batch IEX fraction, it is marked multiple gels.
  4. Note 4: Supplemental Table 2 contains all MALDI TOF data generated for each protein.
  5. Note 5: Only proteins with probability score of 1.0e+000 were included in the table.
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