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Table 1 Proteins whose expression is consistently increased after 8 hour treatment with IGF-1.

From: A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts

Spot Number

Protein Name

NCBI gi #

Fold Increase

SC (%)

Z-score

MW; pI (theoretical)

MW; pI (actual)

 

Adaptor Protein:

      

1

CASK interacting protein 1

161168996

3.8 ± 0.2

11

2.29

141.42/9.5

120/8.5

2

Wbscr1 protein

15928652

3.9 ± 0.4

41

1.93

25.22/7.8

27/7.5

 

Cell surface receptor:

      

3

Stabilin 1

26352640

4.0 ± 0.4

17

2.09

49.21/8.0

55/8.0

 

Cytoskeletal/Structural:

      

4

GRIP and coiled-coil domain-containing protein 2

32469711

3.2 ± 0.1

12

1.75

195.40/5.1

170/5.8

5

Rho interacting protein 3

56205310

3.3 ± 0.1

12

1.27

115.70/6.0

140/6.5

6

Vimentin

55408

5.3 ± 0.6

23

1.93

53.72/5.1

55/5.6

 

DNA Binding protein:

      

7

RAD50

1575575

5.0 ± 0.4

14

1.59

154.61/6.5

80/7.8

 

Enzyme: Hydratase

      

8

Enolase (phosphopyruvate hydratase) (EC 4.2.1.11) alpha

109803

4.7 ± 0.3

29

1.90

47.45/6.4

50/5.7

 

Enzyme: Isomerase

      

9

Triose-phosphate isomerase

1363194

3.8 ± 0.1

27

2.01

27.02/6.9

30/7.5

 

Enzyme: Sulphotransferase:

      

10

N-deacetylase/N-sulfotransferase 4

11344505

3.7 ± 0.3

10

1.74

101.24/6.9

100/6.5

 

Extracellular matrix:

      

11

Collagen alpha-1(XVII) chain

41688459

3.5 ± 0.4

8

1.38

148.48/9.0

160/7.2

 

Heat shock protein (Chaperone):

      

12

Heat shock protein 8

42542422

3.3 ± 0.2

21

1.39

71.08/5.3

78/5.3

13

Heat shock protein A/p66 mot

34784211/5736598

4.1 ± 0.4

14

 

73.80/5.9

62/5.2

 

Phosphotransferase:

      

14

FLT-1 (Tyrosine-protein kinase receptor FLT)

2809069

3.1 ± 0.2

8

1.53

151.71/9.1

160/10

15

Ikbkb protein

22902306

3.6 ± 0.4

22

1.60

87.91/5.7

80/7.8

 

Reductase:

      

16

pyrroline-5-carboxylate reductase-like

119508439

4.1 ± 0.4

27

1.32

29.13/6.8

36/7.9

 

Ribonuclease:

      

17

Plenty-of-prolines-101; POP101; SH3-philo-protein

3153821

5.6 ± 1.0

20

1.68

101.21/11.9

110/6.2

 

Transcription factor:

      

18

NFKB-repressing factor-Transcription factor NRF

12861044

3.0 ± 0.1

13

1.49

77.71/9.1

33/4.5

 

Unclassified/Unknown function:

      

19

Cep290

26353954

4.1 ± 0.3

33

2.08

93.89/7.6

95/7.6

20

mKIAA0209 protein/dedicator of cytokinesis 2

50510415

3.2 ± 0.1

16

1.45

182.35/6.7

125/7.5

21

mKIAA1486 protein

81896693

3.0 ± 0.3

13

1.95

71.19/9.1

63/8.5

22

nasopharyngeal epithelium specific protein 1

 

3.4 ± 0.1

30

2.08

65.98/9.0

75/9.0

23

progesterone-induced blocking factor 1 isoform a

46852193

3.3 ± .03

21

1.73

89.88/5.9

90/6.8

  1. Note 1: Protein classification is according to the Human Protein Reference Database http://www.hprd.org.
  2. Note 2: SC%: Percentage of sequence coverage.
  3. Note 3: Spot Number refers to the location of the individual proteins on 2D gels as shown in Figures 3–6. When a protein was identified in more than one Batch IEX fraction, it is marked multiple gels.
  4. Note 4: Supplemental Table 2 contains all MALDI TOF data generated for each protein.
  5. Note 5: Only proteins with probability score of 1.0e+000 were included in the table.
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