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Figure 3 | Proteome Science

Figure 3

From: Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes

Figure 3

Correlation of immunoblot analysis and 2D-DIGE. A. Immunoblotting was performed as described in the Methods section using lysates from the same cells as for 2D-DIGE. For HSPA1, 5 μg of protein extract was analyzed by 8% SDS-PAGE. For all others, 30 μg extract was analyzed by 14% SDS-PAGE. Immunoblots were developed using a fluorogenic dye (ECF, GE Healthcare). B. Quantification of the immunoblots in Panel A by Kodak 1D 3.5 imaging software. C. Correlation between 2D-DIGE and immunoblot results. Six intergroup comparisons were made. Three were individual comparisons: E6 v. HFK, E7 v. HFK, and E6/7 v. HFK. Three were grouped comparisons: sum of HFK and E6 v. sum of E7 and E6/7, sum of HFK and sum of E7 v. E6 and E6/7, and sum of HFK and E6/7 v. sum of E6 and E7.

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Proteome Science

ISSN: 1477-5956