Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes

Table 1 Significant spots categorized by expression pattern

Trend	x _i	y _i	z _i	n (spots)	n (significant)	Examples
Up-regulated in oncogene-expressing cells	>0	>0	<0	325	57	Oncoprotein DJ-1, ezrin, multiple HSPs, metabolic, and regulatory proteins
	>0	>0	>0	31	5	HSPB1 (2 forms), peroxiredoxin-3
Mixed regulation in oncogene-expressing cells	>0	<0	<0	21	4	HSPA1
	>0	<0	>0	38	0	(none)
	<0	>0	>0	49	3	p16^ink4a, keratin 7 (2 forms)
	<0	>0	<0	69	3	Galectin-7
Down-regulated in oncogene-expressing cells	<0	<0	<0	10	1	(none)
	<0	<0	>0	198	97	Serpin B5, annexin A2, 14-3-3σ, lamin A/C multiple cytokeratins
Total				741	170

All 741 protein spots were classified according to the algebraic sign of x_i, y_i, and z_i. For each of eight possible permutations, the table provides the total number of spots and the number of spots evaluated as potentially significant using statistical and biological criteria specified in Additional file 1. The table also provides examples of identified proteins in each category.

ISSN: 1477-5956