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Table 2 Spots identified by mass spectrometry

From: Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes

Group

spot number

Protein name

Gene name

UniProt Accession Number

DC-1

DC-2

GC-C

DC-3

     

2xi

2yi

2zi

2wi

Down-regulated 2xi< 1, 2yi< 1

572

Keratin, type II cytoskeletal 6C

KRT6C

P48668

0.32

0.19

4.47

0.27

 

560

Keratin, type II cytoskeletal 6C

KRT6C

P48668

0.35

0.21

4.13

0.3

 

545

Pyruvate kinase isozyme M2

PKM2

P14618

0.37

0.14

7.14

0.36

 

1110

Annexin A2b

ANXA2

Q8TBV2

0.38

0.66

1.36

0.34

 

775

ATP synthase subunit b, mitochondrial

ATP5B

P06576

0.38

0.13

7.79

0.39

 

2967

Keratin, type II cytoskeletal 6A

KRT6A

P02538

0.39

0.27

3.12

0.33

 

672

Keratin, type I cytoskeletal 14

KRT14

P02533

0.4

0.27

3.09

0.33

 

1111

Annexin A2b (lipocortin)

ANXA2

Q8TBV2

0.41

0.68

1.39

0.39

 

439

Progerin (Lamin A/C)

LMNA

Q6UYC3

0.41

0.54

2.77

0.62

 

534

Keratin, type II cytoskeletal 6A

KRT6A

P02538

0.43

0.22

4.5

0.42

 

460

Pyruvate kinase isozyme M2

PKM2

P14618

0.46

0.58

2.58

0.69

 

739

Keratin, type II cytoskeletal 8

KRT8

P05787

0.5

0.3

3.49

0.53

 

1586

14-3-3 protein σ (stratifin)

SFN

P31947

0.53

0.49

1.82

0.47

 

903

Serpin B5 (maspin)

PI5

P36952

0.7

0.45

1.6

0.51

 

915

Keratin, type I cytoskeletal 18

KRT18

P05783

0.76

0.48

1.75

0.65

Mixed regulation 2xi< 1, 2yi> 1, OR 2xi> 1, 2yi< 1

2402

Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 (p16)

CDKN2A

P42771

0.34

4.83

3.46

5.77

 

645

Keratin, type II cytoskeletal 7c

KRT7

P08729

0.37

2.32

1.53

1.31

 

646

Keratin, type II cytoskeletal 7

KRT7

P08729

0.3

1.6

2.06

0.97

 

2597

Galectin-7

LGALS7

P47929

0.37

2.51

0.25

0.23

 

366

HSPA1A

HSPA1A

P08107

3.01

0.86

0.71

1.86

Up-regulated 2xi> 1, 2yi> 1

1451

EF-hand domain containing protein D2

EFHD2

Q96C19

1.17

2.06

0.59

1.42

 

266

Ezrin (villin-2)

VIL2

P15311

1.5

2.55

0.45

1.7

 

1685

HSPB1 (Hsp27)

HSPB1

P04792

2

1.14

0.44

1.01

 

2983

Elongation factor 1-δ

EEF1D

P29692

2.08

1.84

0.68

2.58

 

1186

Inorganic pyrophosphatase

PPA1

Q15181

2.26

1.4

0.58

1.82

 

1721

Keratin, type I cytoskeletal 10

KRT10

P13645

2.34

2.58

0.26

1.56

 

1694

HSPB1

HSPB1

P04792

2.48

1.08

0.47

1.25

 

777

α-enolase

ENO1

P06733

2.52

1.25

0.41

1.3

 

781

α-enolase

ENO1

P06733

2.57

1.27

0.34

1.12

 

778

α-enolase

ENO1

P06733

2.6

1.28

0.34

1.15

 

1849

Protein DJ-1

PARK7

Q99497

2.79

1.43

0.31

1.22

 

382

HSPA9 (mortalin, GRP 75)

HSPA9

P38646

2.92

1.29

0.31

1.17

 

766

α-enolase

ENO1

P06733

3.27

1.49

0.26

1.25

 

1859

Protein DJ-1

PARK7

Q99497

3.93

1.58

0.23

1.45

 

377

HSPA1A (Hsp70-1)

HSPA1A

P08107

4.08

1.65

0.21

1.4

 

1678

HSPB1 (Hsp27)

HSPB1

P04792

6.95

1.13

0.24

1.86

 

1686

HSPB1 (Hsp27)

HSPB1

P04792

1.04

1.45

1.91

2.88

 

1839

Thioredoxin-dependent peroxide reductase, mitochondrialc

PRDX3

P30048

1.66

1.54

1.01

2.58

 

1663

HSPB1c (Hsp27)

HSPB1

P04792

4.04

1.59

2.8

17.97

  1. DC-1, DC-2, and DC-3 stand for direct comparisons 1, 2, and 3. GC-C stands for grouped comparison C. All proteins met criteria for significance. bSignificant in group comparison A. c Significant in both group comparisons A and B. Proteins are grouped by the direction of expression for the viral oncogene-transduced cultures as compared to non-viral oncogene-expressing primary HFKs. Names and accession numbers are in the UniProt format. Note that by definition, z i = w i . - (x i + y i ). For this table, the x i , y i , z i , and w i values have been exponentiated (i.e., converted to fold change) to facilitate interpretation. Proteins with values < 0.5 and > 2.0 represent a 2-fold change (absolute value of x i or y i > 1). Group symbols correlate with the symbols used in Figure 4.
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Proteome Science

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