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Table 2 Spots identified by mass spectrometry

From: Large-scale analysis of protein expression changes in human keratinocytes immortalized by human papilloma virus type 16 E6 and E7 oncogenes

Group spot number Protein name Gene name UniProt Accession Number DC-1 DC-2 GC-C DC-3
      2xi 2yi 2zi 2wi
Down-regulated 2xi< 1, 2yi< 1 572 Keratin, type II cytoskeletal 6C KRT6C P48668 0.32 0.19 4.47 0.27
  560 Keratin, type II cytoskeletal 6C KRT6C P48668 0.35 0.21 4.13 0.3
  545 Pyruvate kinase isozyme M2 PKM2 P14618 0.37 0.14 7.14 0.36
  1110 Annexin A2b ANXA2 Q8TBV2 0.38 0.66 1.36 0.34
  775 ATP synthase subunit b, mitochondrial ATP5B P06576 0.38 0.13 7.79 0.39
  2967 Keratin, type II cytoskeletal 6A KRT6A P02538 0.39 0.27 3.12 0.33
  672 Keratin, type I cytoskeletal 14 KRT14 P02533 0.4 0.27 3.09 0.33
  1111 Annexin A2b (lipocortin) ANXA2 Q8TBV2 0.41 0.68 1.39 0.39
  439 Progerin (Lamin A/C) LMNA Q6UYC3 0.41 0.54 2.77 0.62
  534 Keratin, type II cytoskeletal 6A KRT6A P02538 0.43 0.22 4.5 0.42
  460 Pyruvate kinase isozyme M2 PKM2 P14618 0.46 0.58 2.58 0.69
  739 Keratin, type II cytoskeletal 8 KRT8 P05787 0.5 0.3 3.49 0.53
  1586 14-3-3 protein σ (stratifin) SFN P31947 0.53 0.49 1.82 0.47
  903 Serpin B5 (maspin) PI5 P36952 0.7 0.45 1.6 0.51
  915 Keratin, type I cytoskeletal 18 KRT18 P05783 0.76 0.48 1.75 0.65
Mixed regulation 2xi< 1, 2yi> 1, OR 2xi> 1, 2yi< 1 2402 Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 (p16) CDKN2A P42771 0.34 4.83 3.46 5.77
  645 Keratin, type II cytoskeletal 7c KRT7 P08729 0.37 2.32 1.53 1.31
  646 Keratin, type II cytoskeletal 7 KRT7 P08729 0.3 1.6 2.06 0.97
  2597 Galectin-7 LGALS7 P47929 0.37 2.51 0.25 0.23
  366 HSPA1A HSPA1A P08107 3.01 0.86 0.71 1.86
Up-regulated 2xi> 1, 2yi> 1 1451 EF-hand domain containing protein D2 EFHD2 Q96C19 1.17 2.06 0.59 1.42
  266 Ezrin (villin-2) VIL2 P15311 1.5 2.55 0.45 1.7
  1685 HSPB1 (Hsp27) HSPB1 P04792 2 1.14 0.44 1.01
  2983 Elongation factor 1-δ EEF1D P29692 2.08 1.84 0.68 2.58
  1186 Inorganic pyrophosphatase PPA1 Q15181 2.26 1.4 0.58 1.82
  1721 Keratin, type I cytoskeletal 10 KRT10 P13645 2.34 2.58 0.26 1.56
  1694 HSPB1 HSPB1 P04792 2.48 1.08 0.47 1.25
  777 α-enolase ENO1 P06733 2.52 1.25 0.41 1.3
  781 α-enolase ENO1 P06733 2.57 1.27 0.34 1.12
  778 α-enolase ENO1 P06733 2.6 1.28 0.34 1.15
  1849 Protein DJ-1 PARK7 Q99497 2.79 1.43 0.31 1.22
  382 HSPA9 (mortalin, GRP 75) HSPA9 P38646 2.92 1.29 0.31 1.17
  766 α-enolase ENO1 P06733 3.27 1.49 0.26 1.25
  1859 Protein DJ-1 PARK7 Q99497 3.93 1.58 0.23 1.45
  377 HSPA1A (Hsp70-1) HSPA1A P08107 4.08 1.65 0.21 1.4
  1678 HSPB1 (Hsp27) HSPB1 P04792 6.95 1.13 0.24 1.86
  1686 HSPB1 (Hsp27) HSPB1 P04792 1.04 1.45 1.91 2.88
  1839 Thioredoxin-dependent peroxide reductase, mitochondrialc PRDX3 P30048 1.66 1.54 1.01 2.58
  1663 HSPB1c (Hsp27) HSPB1 P04792 4.04 1.59 2.8 17.97
  1. DC-1, DC-2, and DC-3 stand for direct comparisons 1, 2, and 3. GC-C stands for grouped comparison C. All proteins met criteria for significance. bSignificant in group comparison A. c Significant in both group comparisons A and B. Proteins are grouped by the direction of expression for the viral oncogene-transduced cultures as compared to non-viral oncogene-expressing primary HFKs. Names and accession numbers are in the UniProt format. Note that by definition, z i = w i . - (x i + y i ). For this table, the x i , y i , z i , and w i values have been exponentiated (i.e., converted to fold change) to facilitate interpretation. Proteins with values < 0.5 and > 2.0 represent a 2-fold change (absolute value of x i or y i > 1). Group symbols correlate with the symbols used in Figure 4.
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