Figure 3

Negative images of Enterobacter sp. YSU total protein separated by 2DGE over a pI range of 4-7. Cultures were grown as in Fig 2. Cultures grown with No L-Cysteine and No Selenite (NCNS) and with L-Cysteine and No Selenite (CNS) were harvested after 2.5 hours of growth. The cultures grown with No L-Cysteine and Selenite (NCS) and with L-Cysteine and Selenite (CS) were harvested after 3.5 hours of growth. Spots identified with blue arrows appeared with equal intensities under all 4 conditions. The spot identified by a purple arrow appeared at a higher intensity in gels containing samples from cells grown in the absence of L-cysteine. Spots identified by dark red arrows appeared at higher intensities in gels containing samples from cells grown in the absence of selenite. Spots identified by red arrows appeared at higher intensities in gels containing samples from cells grown in the presence of selenite. The spot numbers correspond to the spot numbers in Table 1.