Figure 4

Detection of the cysK , cysA , cysT , cysW and cysE transcripts using RT-PCR. Cells were grown and harvested as in Fig 3. Equal volumes of cDNA synthesized from 0.5 μg of total RNA were used in PCR reactions containing primers specific for each gene, and 10 μl of each PCR reaction were analyzed by agarose gel electrophoresis. Lanes: (1) No L-Cysteine, No Selenite (NCNS); (2) No L-Cysteine, Selenite (NCS); (3) L-Cysteine, No Selenite (CNS); (4) L-Cysteine, Selenite (CS). The gene, cysE, was used as an internal control.