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Table 1 Identification of select protein spots that appeared under all four conditions (NCNS, NCS, CNS and CS)a.

From: Proteomic profiling of L-cysteine induced selenite resistance in Enterobacter sp. YSU

Spot #/Condition

Protein Name

NCBI Accession (Version)

Theo. Mr/pI (kDa)

Estimated Expt. Mr/pI (kDa)

Species

Mascot Score

NP/PD

MS/MS Peptide Sequence

SC (%)

1

Equally intense under all conditions

OmpF porin

CAC48383

gi|15131544

4.63/38.4

4.6/30

Enterobacter cloacae

520

8/31

DGNKLDLYGK

LDLYGK

LAFAGLK

FGDAGSFDYGR

TGGLATYR

AEQWATGLK

YDANNIYLAALYGEMR

NMSTYVDYQINQLKDDNK

22%

2

Equally intense under all conditions

protein chain elongation factor EF-Ts

AAL19181

gi|16418721

5.13/30.4

5.3/25

Salmonella typhimurium

1090

13/69

AEITASLVKELRER

ALTEANGDIELAIENMRK

KAGNVAADGVIKTK

DAGFQAFADK

VLDAAVAGK

ITDVEVLK

AQFEEER

IGENINIR

GADEELVK

EYQVQLDIAMQSGKPK

EHNADVTGFIRFEVGEGIEKVETDFAAEVAAMSK

FEVGEGIEKVETDFAAEVAAMSK

VETDFAAEVAAMSK

51%

3

NCNS & NCS

conserved hypothetical lipobinding protein

NP_758751

gi|27228700

5.57/29.5

6/32

Erwinia pyrifoliae

153

2/9

ISDIVENPK

YKGAVIPVNN

7%

Only in NCS

branched-chain amino-acid aminotransferase

AAM83928

gi|21957021

6.22/36.9

6/32

Yersinia pestis

277

5/6

IYRMPVSQSVDELMEACR

VAPNTIPTAAK

AGGNYLSSLLVGSEAR

DGILFTPPFTSSALPGITR

ESLYLADEVFMSGTAAEITPVR

25%

Only in NCS

outer membrane protein II

AAA24807

gi|148368

4.88/25.7

6/32

Enterobacter aerogenes

104

2/3

LGYPVTDDLDVYTR

SDVLFNFNK

9%

4

NCNS & NS

translation elongation factor EF-Tu

AAL22974

gi|16422703

5.24/43.5

5.8/35

Salmonella typhimurium

1143

18/64

TTLTAAITTVLAK

AFDQIDNAPEEK

GITINTSHVEYDTPTR

NMITGAAQMDGAILVVAATDGPMPQTR

EHILLGR

QVGVPYIIVFLNK

CDMVDDEELLELVEMEVR

ELLSQYDFPGDDTPIVR

AIDKPFLLPIEDVFSISGR

VGEEVEIVGIK

STCTGVEMFR

KLLDEGR

AGENVGVLLR

FESEVYILSK

GYRPQFYFR

TTDVTGTIELPEGVEMVMPGDNIK

MVVTLIHPIAMDDGLR

TVGAGVVAK

64%

5, 6

NCNS & NS

translation elongation factor EF-Tu

AAL22974

gi|16422703

5.3/43.4

5.8/36

Salmonella typhimurium

666

10/19

TTLTAAITTVLAK

AFDQIDNAPEEK

NMITGAAQMDGAILVVAATDGPMPQTR

ELLSQYDFPGDDTPIVR

VGEEVEIVGIK

AGENVGVLLR

FESEVYILSK

GYRPQFYFR

MVVTLIHPIAMDDGLR

TVGAGVVAK

30%

In 6 but not in 5

5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase

CAG76025

gi|49612575

5.69/38.9

5.8/36

Erwinia carotovora

173

4/6

ILNQEAR

LAWEFAK

ALQFVDADK

AGIDIVSDGEQTR

10%

7, 10, 11

NCS & CS

outer membrane protein II

AAA24807

gi|148368

4.88/25.6

5.1/35

Enterobacter aerogenes

921

11/64

LGYPVTDDLDVYTRLGGMVWR

ADTSNSIAGDDHDTGVSPVFAGGVEWAMTR

LEYQWVNNIGDGATVGVRPDNGMLSVGVSYR

FGQQEDAPVVAPAPAPAPEVQTK

SDVLFNFNK

ATLKPEGQQALDQLYTQLSNLDPK

DGSVVVLGFTDR

IGSDAYNQGLSEKR

RAQSVVDYLVSK

GMGESNPVTGSTCDNVKPR

AALIDCLAPDR

86%

NCS & CS

OmpA

AAY18798

gi|62901665

5.19/37.1

5.1/35

Enterobacter sakazakii

700

10/54

DNTWYAGGK

AQGVQLTAK

LGYPVTDDLDVYTRLGGMVWR

LGGMVWR

ADSSSNIAGDDHDTGVSPVFAGGVEWAMTRDIAT SDVLFNFNK

DGSVVVLGFTDR

IGSDAYNQGLSEKR

AQSVVDYLISK

GMGESNPVTGNTCDNVKAR

40%

NCS & CS

putative membrane component hydrogenase

AAL20003

gi|16419585

5.6/37.6

5.1/35

Salmonella typhimurium

561

8/45

AQGVQLTAK

LGYPITDDLDVYTR

LGGMVWR

SDVLFNFNK

DGSVVVLGFTDR

IGSDAYNQGLSEKR

AQSVVDYLISK

AALIDCLAPDRR

25%

Only in spot 7, NCS

cysK protein

AAA23654

gi|145686

5.64/34.6

5.1/35

Escherichia coli

245

4/9

LTLTMPETMSIER

ALGANLVLTEGAK

VIGITNEEAISTAR

NIVVILPSSGER

16%

Only in spot 10

60 kDa chaperonin (groEL)

O66200

gi|6225120

4.85/56.5

5/35

Pantoea agglomerans

333

4/6

ANDAAGDGTTTATVLAQAIITEGLK

AIAQVGTISANSDETVGK

AMLQDIATLTGGTVISEEIGMELEK

LAGLTAQNEDQNVGIK

15%

8

Only in NCS

putative tellurium resistance protein C

AAF36435

gi|7108483

4.56/20.5

4.8/22

Escherichia coli

516

6/14

AAPSMKNVLVGLGWDAR

GDSDFIFYNNLTSSDGSVTHTGDNR

TGEGDGDDESLKIK

RQSFGQVSGAFIR

LVNDDNQTEVAR

YDLTEDASTETAMLFGELYR

52%

 

similar to GroES protein

BAA25224

gi|2980925

4.90/9.3

4.8/22

Klebsiella pneumoniae

184

3/5

SAGGIVLTGSAAAK

ILENGTVQPLDVK

VGDIVIFNDGYGVK

46%

 

outer membrane protein II

AAA24807

gi|148368

4.88/25.6

4.8/22

Enterobacter aerogenes

191

2/6

LGYPVTDDLDVYTRLGGMVWR

FGQQEDAPVVAPAPAPAPEVQTK

18%

 

OmpA

AAY18798

gi|62901665

5.19/37.1

4.8/22

Enterobacter sakazakii

165

2/4

AQGVQLTAK

LGYPVTDDLDVYTRLGGMVWR

8%

9

Only in NCS

small heat shock protein

AAL22668

gi|16422381

5.23/15.7

6.5/10

Salmonella typhimurium

299

4/26

MRNFDLSPLYR

SAIGFDR

ERTYLYQGIAER

GANLVNGLLYIELER

32%

  1. a Spot number corresponds to labeled spots in Fig 3; Condition refers to the gel(s) in which the spot or protein appeared at the greatest intensity; Protein name, matched protein description; NCBI Accession (Version), accession number and submission version of matched protein from NCBI database; Theo. pI/Mr (kDa), theoretical isoelectric point and molecular mass based on amino acid sequence of the identified protein; Expt. pI/Mr (kDa), experimental isoelectric point and molecular mass estimated from the 2DGE gels; Species, the bacterial species of the matched protein; Mascot score, score obtained from the Mascot search for each match; NP, the number of matched peptides; PD, the number of peptides detected; MS/MS Peptide Sequence, amino acid sequences of peptides identified by Nano-LC/MS/MS; SC, percent amino acid sequence coverage for the identified protein.