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Figure 4 | Proteome Science

Figure 4

From: Transchromosomic cell model of Down syndrome shows aberrant migration, adhesion and proteome response to extracellular matrix

Figure 4

Representative SELDI-TOF MS spectra from A9 samples. (A) A9 and WA17 cells were lysed and proteins resolved by SDS-PAGE. Equal amounts of total protein lysate were used for electrophoresis (20 μg). (B) An example of SELDI protein-profiling spectra of 2 μg of whole cell protein extract of A9 cell line on four different chromatographic arrays (H50, IMAC, Q10 and CM10) is shown. (C) A dilution series of A9 cell protein extract sample was prepared and 2 μl of each was analyzed on a Q10 and CM10 ProteinChip array. The total amount of protein analyzed was 2, 1, 0.5 and 0.1 μg, respectively.

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Proteome Science

ISSN: 1477-5956