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Table 1 Significantly up-regulated proteins identified in the plasma membrane fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

     theoretical experimental    
spot# orf protein name %cov pI MW (kDa) pI MW (kDa) fold* t-test cluster t
Up regulated proteins (separated by pH range 3-10 in the first dimension)          
Two component systems
357 AP07650006 Two-component hybrid sensor and regulator 6.47 4.97 146.95 5.71 265.61 1.43 0.044 5
518 AP07580004 Two-component sensor histidine kinase 8.57 5.26 133.55 5.36 239.78 1.78 0.0059 23
793 AP06360005 Two component hybrid sensor and regulator 6.9 5.41 159.88 6.4 188.54 2.47 0.013 14
808 AP07880008 Two-component hybrid sensor and regulator 2.86 4.97 200.24 6.17 183.78 3.15 0.025 15
872 AP07580004 Two-component sensor histidine kinase 5.94 5.26 133.55 5.28 174.61 1.89 0.027 23
1097 AP06990006 Hybrid sensor and regulator 7.34 5.5 104.01 5.88 140.14 1.43 0.047 23
1102 AP07670017 Two-component sensor histidine kinase 6.57 4.88 124.16 4.58 139.07 1.96 0.034 23
1147 AP07670017 Two-component sensor histidine kinase 8.51 4.88 124.16 4.65 130.45 1.77 0.041 23
1241 AP07670017 Two-component sensor histidine kinase 5.92 4.88 124.16 4.48 120.51 3.49 0.019 15
1564 AP07970028 Two-component system sensory histidine kinase 6.22 4.8 108.09 4.49 90.03 1.71 0.016 23
1592 AP04840005 Serine/threonine kinase with TPR repeat 7.45 8.37 82.39 5.24 86.42 1.81 0.043 23
2406 AP07830002 Sensory box/GGDEF family protein 8.15 5.38 53.01 4.51 39.31 1.78 0.016 9
Stress related proteins
508 AP06620005 Glycosyl transferase, family 2:Glycosyl transferase, group 1 4.14 6.57 128.59 5.29 239.78 1.82 0.025 23
526 AP06740013 Ferredoxin-glutamate synthase 5.87 5.6 169.95 5.43 238.56 2.2 0.042 14
532 AP05380002 DEAD/DEAH box helicase domain protein (membrane-helicase) 5.29 6.09 239.75 5.5 236.73 2.24 0.0014 14
821 AP05300003 ATPase of the ABC class 9.74 5.46 66.65 6.63 184.25 2.18 0.0093 23
874p AP05380002 DEAD/DEAH box helicase domain protein (membrane-helicase) 4.81 6.09 239.75 5.48 175.06 1.95 0.013 23
995 AP07470001 RNA polymerase sigma-70 factor 19.07 9.81 30.04 5.56 154.44 1.76 0.024 23
1028 AP06740013 Ferredoxin-glutamate synthase 6.89 5.6 169.95 4.47 151.31 2.09 0.011 23
1029 AP08060123 Putative transcriptional regulator, LysR family 4.49 6.61 34.76 4.57 148.63 2.11 0.035 23
1277 AP05380002 DEAD/DEAH box helicase domain protein (membrane-helicase) 3.9 6.09 239.75 5.48 116.27 1.99 0.026 23
1284 AP06510003 Putative membrane carboxypeptidase 5.77 8.68 84.38 5.68 115.68 1.65 0.039 23
1388 AP06740013 Ferredoxin-glutamate synthase 4.47 5.6 169.95 7.08 105.50 2 0.023 18
2187 AP04600003 S-adenosyl-L-homocysteine hydrolase 10.27 5.63 48.77 4.52 48.24 1.57 0.037 9
DNA damage/DNA repairing system          
1479 AP07790020 Type II site-specific deoxyribonuclease 5.03 6.85 35.22 6.53 97.71 3.88 0.021 15
1547 AP02010002 Restriction endonuclease 11.57 5.26 53.91 5.66 91.66 1.69 0.031 14
Proteins containing conserved motifs          
422 AP07330005 TPR repeat- peptidase M, neutral zinc metallopeptidase, zinc-binding site 4.54 8.84 95.92 3.65 253.01 1.38 0.02 9
571 AP06390003 WD-40 repeat protein- transcriptional regulator, XRE family 5.52 6.2 160.34 3.48 222.64 3.11 0.048 22
943 AP07740012 TPR repeat-glycosyl transferase/capsule polysaccharide biosynthesis 2.95 5.59 103.58 5.27 161.30 1.99 0.032 14
1274 AP05550005 TPR repeat-putative prenyltransferase/glycosyl transferase 12.7 5.34 41.12 4.58 118.07 1.45 0.025 9
1817 AP06930009 TPR repeat-O-linked GlcNAc transferase 3.65 5.94 71.42 6.33 70.07 1.81 0.018 23
1983 AP05550005 TPR repeat-putative prenyltransferase/glycosyl transferase 12.7 5.34 41.12 6.35 61.19 1.8 0.013 14
2733p AP05550005 TPR repeat-putative prenyltransferase/glycosyl transferase 12.7 5.34 41.12 4.93 28.19 1.74 0.049 9
Others          
611 AP06080002 Putative enzyme of poly-gamma-glutamate biosynthesis, capsule formation 5.68 5.59 72.57 5.83 221.50 1.9 0.031 14
Up regulated proteins (separated by pH range 4-7 in the first dimension)          
Two component system          
2386 AP01720004 Putative two-component sensor histidine kinase 6.34 5.25 57.98 5.08 53.46 2.21 0.038 18
Stress related proteins          
1569 AP05860006 Putative glycosyl transferase 9.08 5.62 132.60 4.84 96.48 1.89 0.031 23
2179 AP07990044 Putative ABC transporter 6.92 8.57 88.65 5.74 62.93 2.4 0.011 14
Translation machinery          
1477 AP07620038 50S ribosomal protein L4 13.81 10.04 23.34 5.08 103.76 2.81 0.02 14
  1. * Fold represents fold change value which is volume ratio of after the temperature upshift (180 min)/before the temperature upshift. Volume ratio refers to the ratio of the normalized volumes of a pair of spots (the same spot of before and after the temperature upshift), for example, a value of 2.0 represents a two-fold increase while -2.0 represents a two-fold decrease.
  2. p These protein spots are phosphorylated at Ser, Thr and Tyr residues, detected by western blot analysis before the temperature upshift (0 min) and after the temperature upshift (45, 90, 180 min).
  3. t Cluster types; (i) cluster 5, 14,15,18 and 23 are sustained tolerance proteins (ii) cluster 22 is adaptation proteins and (iii) cluster 9 and 14 are undetermined-pattern proteins.
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