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Table 1 Significantly up-regulated proteins identified in the plasma membrane fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

    

theoretical

experimental

   

spot#

orf

protein name

%cov

pI

MW (kDa)

pI

MW (kDa)

fold*

t-test

cluster t

Up regulated proteins (separated by pH range 3-10 in the first dimension)

         

Two component systems

357

AP07650006

Two-component hybrid sensor and regulator

6.47

4.97

146.95

5.71

265.61

1.43

0.044

5

518

AP07580004

Two-component sensor histidine kinase

8.57

5.26

133.55

5.36

239.78

1.78

0.0059

23

793

AP06360005

Two component hybrid sensor and regulator

6.9

5.41

159.88

6.4

188.54

2.47

0.013

14

808

AP07880008

Two-component hybrid sensor and regulator

2.86

4.97

200.24

6.17

183.78

3.15

0.025

15

872

AP07580004

Two-component sensor histidine kinase

5.94

5.26

133.55

5.28

174.61

1.89

0.027

23

1097

AP06990006

Hybrid sensor and regulator

7.34

5.5

104.01

5.88

140.14

1.43

0.047

23

1102

AP07670017

Two-component sensor histidine kinase

6.57

4.88

124.16

4.58

139.07

1.96

0.034

23

1147

AP07670017

Two-component sensor histidine kinase

8.51

4.88

124.16

4.65

130.45

1.77

0.041

23

1241

AP07670017

Two-component sensor histidine kinase

5.92

4.88

124.16

4.48

120.51

3.49

0.019

15

1564

AP07970028

Two-component system sensory histidine kinase

6.22

4.8

108.09

4.49

90.03

1.71

0.016

23

1592

AP04840005

Serine/threonine kinase with TPR repeat

7.45

8.37

82.39

5.24

86.42

1.81

0.043

23

2406

AP07830002

Sensory box/GGDEF family protein

8.15

5.38

53.01

4.51

39.31

1.78

0.016

9

Stress related proteins

508

AP06620005

Glycosyl transferase, family 2:Glycosyl transferase, group 1

4.14

6.57

128.59

5.29

239.78

1.82

0.025

23

526

AP06740013

Ferredoxin-glutamate synthase

5.87

5.6

169.95

5.43

238.56

2.2

0.042

14

532

AP05380002

DEAD/DEAH box helicase domain protein (membrane-helicase)

5.29

6.09

239.75

5.5

236.73

2.24

0.0014

14

821

AP05300003

ATPase of the ABC class

9.74

5.46

66.65

6.63

184.25

2.18

0.0093

23

874p

AP05380002

DEAD/DEAH box helicase domain protein (membrane-helicase)

4.81

6.09

239.75

5.48

175.06

1.95

0.013

23

995

AP07470001

RNA polymerase sigma-70 factor

19.07

9.81

30.04

5.56

154.44

1.76

0.024

23

1028

AP06740013

Ferredoxin-glutamate synthase

6.89

5.6

169.95

4.47

151.31

2.09

0.011

23

1029

AP08060123

Putative transcriptional regulator, LysR family

4.49

6.61

34.76

4.57

148.63

2.11

0.035

23

1277

AP05380002

DEAD/DEAH box helicase domain protein (membrane-helicase)

3.9

6.09

239.75

5.48

116.27

1.99

0.026

23

1284

AP06510003

Putative membrane carboxypeptidase

5.77

8.68

84.38

5.68

115.68

1.65

0.039

23

1388

AP06740013

Ferredoxin-glutamate synthase

4.47

5.6

169.95

7.08

105.50

2

0.023

18

2187

AP04600003

S-adenosyl-L-homocysteine hydrolase

10.27

5.63

48.77

4.52

48.24

1.57

0.037

9

DNA damage/DNA repairing system

         

1479

AP07790020

Type II site-specific deoxyribonuclease

5.03

6.85

35.22

6.53

97.71

3.88

0.021

15

1547

AP02010002

Restriction endonuclease

11.57

5.26

53.91

5.66

91.66

1.69

0.031

14

Proteins containing conserved motifs

         

422

AP07330005

TPR repeat- peptidase M, neutral zinc metallopeptidase, zinc-binding site

4.54

8.84

95.92

3.65

253.01

1.38

0.02

9

571

AP06390003

WD-40 repeat protein- transcriptional regulator, XRE family

5.52

6.2

160.34

3.48

222.64

3.11

0.048

22

943

AP07740012

TPR repeat-glycosyl transferase/capsule polysaccharide biosynthesis

2.95

5.59

103.58

5.27

161.30

1.99

0.032

14

1274

AP05550005

TPR repeat-putative prenyltransferase/glycosyl transferase

12.7

5.34

41.12

4.58

118.07

1.45

0.025

9

1817

AP06930009

TPR repeat-O-linked GlcNAc transferase

3.65

5.94

71.42

6.33

70.07

1.81

0.018

23

1983

AP05550005

TPR repeat-putative prenyltransferase/glycosyl transferase

12.7

5.34

41.12

6.35

61.19

1.8

0.013

14

2733p

AP05550005

TPR repeat-putative prenyltransferase/glycosyl transferase

12.7

5.34

41.12

4.93

28.19

1.74

0.049

9

Others

         

611

AP06080002

Putative enzyme of poly-gamma-glutamate biosynthesis, capsule formation

5.68

5.59

72.57

5.83

221.50

1.9

0.031

14

Up regulated proteins (separated by pH range 4-7 in the first dimension)

         

Two component system

         

2386

AP01720004

Putative two-component sensor histidine kinase

6.34

5.25

57.98

5.08

53.46

2.21

0.038

18

Stress related proteins

         

1569

AP05860006

Putative glycosyl transferase

9.08

5.62

132.60

4.84

96.48

1.89

0.031

23

2179

AP07990044

Putative ABC transporter

6.92

8.57

88.65

5.74

62.93

2.4

0.011

14

Translation machinery

         

1477

AP07620038

50S ribosomal protein L4

13.81

10.04

23.34

5.08

103.76

2.81

0.02

14

  1. * Fold represents fold change value which is volume ratio of after the temperature upshift (180 min)/before the temperature upshift. Volume ratio refers to the ratio of the normalized volumes of a pair of spots (the same spot of before and after the temperature upshift), for example, a value of 2.0 represents a two-fold increase while -2.0 represents a two-fold decrease.
  2. p These protein spots are phosphorylated at Ser, Thr and Tyr residues, detected by western blot analysis before the temperature upshift (0 min) and after the temperature upshift (45, 90, 180 min).
  3. t Cluster types; (i) cluster 5, 14,15,18 and 23 are sustained tolerance proteins (ii) cluster 22 is adaptation proteins and (iii) cluster 9 and 14 are undetermined-pattern proteins.
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