Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

Hongsthong, Apiradee; Sirijuntarut, Matura; Yutthanasirikul, Rayakorn; Senachak, Jittisak; Kurdrid, Pavinee; Cheevadhanarak, Supapon; Tanticharoen, Morakot

doi:10.1186/1477-5956-7-33

Table 2 Significantly up-regulated proteins identified in the soluble fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

				theoretical		experimental
spot#	orf	protein name	%cov	pI	MW (kDa)	pI	MW (kDa)	fold*	t-test	cluster ^t
Up regulated proteins (separated by pH range 3-10 in the first dimension)
Two component systems
1282	AP07350018	Hybrid sensor and regulator	4.51	5.16	157.04	9.24	125.42	5.21	0.047	22
1399	AP08000025	Two-component sensor histidine kinase	8.04	5.09	80.65	8.67	116.52	3.42	0.022	4
2377	AP07580004	Two-component sensor histidine kinase	7.38	5.26	133.55	7.69	51.45	1.51	0.012	16
2765	AP07580004	Two-component sensor histidine kinase	4.07	5.26	133.55	8.13	34.68	4.05	0.0035	4
Stress related proteins
2693	AP04260002	NADPH-dependent FMN reductase	7.48	6.53	24.75	3.37	37.83	2.98	0.039	5
Translation machinery
23	AP07220011	30S ribosomal protein S1	14.32	4.48	42.60	4.88	308.16	1.55	0.037	16
Hypothetical proteins
410	AP07020011	Conserved hypothetical protein	2.74	5.46	249.83	3.61	228.97	2.86	0.026	1
Proteins containing conserved motif
1736	AP06120011	TPR repeat-containing protein-O-linked GlcNAc transferase	5.79	4.88	72.71	3.76	91.01	1.54	0.05	16
Up regulated proteins (separated by pH range 4-7 in the first dimension)
Two component systems
599^p	AP03710004	Sensory box/GGDEF family protein	11.55	4.95	65.15	5.16	226.88	4.41	0.034	4
665	AP06710002	Multi-sensor signal transduction histidine kinase	5.45	5.25	116.20	5.03	220.07	1.75	0.047	6
727	AP07310007	Hybrid sensory kinase	2.82	5.34	100.91	5.13	209.99	1.87	0.039	19
735	AP06460007	Multi-sensor Hybrid Histidine Kinase	6.58	4.93	200.70	5.21	210.48	1.73	0.039	1
737	AP02950002	Putative response regulator receiver signal transduction histidine kinase	13.19	4.91	42.67	5.27	209.99	2.04	0.0073	10
788	AP08040014	Ethylene response sensor protein	6.1	4.92	120.25	5.32	203.21	5.64	0.0035	2
1072	AP04840005	Serine/threonine kinase with TPR repeat	7.03	8.37	82.39	5.92	172.45	1.57	0.026	6
1153	AP07580004	Two-component sensor histidine kinase	5.85	5.26	133.55	5.75	159.61	1.54	0.025	6
1343	AP07350018	Hybrid sensor and regulator	2.25	5.16	157.04	5.73	142.96	1.34	0.02	6
1357	AP07580004	Two-component sensor histidine kinase	2.37	5.26	133.55	4.9	139.65	3.73	0.0091	4
1377	AP07580004	Two-component sensor histidine kinase	5.51	5.26	133.55	5.21	137.37	3.29	0.022	5
1482	AP07670017	Two-component sensor histidine kinase	4.07	4.88	124.16	5.59	127.74	1.7	0.021	6
1787	AP07430015	Sensory box histidine kinase/response regulator	1.21	5.41	138.91	4.56	102.00	2.01	0.0012	19
1883^p	AP06710002	Multi-sensor signal transduction histidine kinase	5.74	5.25	116.20	5.94	94.40	1.48	0.021	6
2635	AP04840005	Serine/threonine kinase with TPR repeat	5.79	8.37	82.39	5.31	50.01	1.36	0.016	6
2940	AP08000025	Two-component sensor histidine kinase	4.23	5.09	80.65	5.2	38.73	1.42	0.035	6
Stress related proteins
460	AP07250004	Glycosyl transferase, family 2	1.19	5.45	236.54	5.34	248.02	2.16	0.0088	8
512^p	AP07620006	ABC transporter-like protein	8.96	5.5	64.24	5.3	238.33	3.11	0.01	4
613	AP07180022	Putative aldehyde dehydrogenase	12.21	6.38	29.30	5.36	226.35	4.15	0.021	4
799	AP06960005	Fe-S oxidoreductase	4.58	5.83	60.23	5.21	202.26	4.87	0.017	4
1328	AP07510011	Ribitol type dehydrogenase protein	5.1	5.53	47.30	5.96	143.97	2.11	0.013	1
1753	AP06510003	Putative membrane carboxypeptidase	3.15	8.68	84.38	5.46	105.40	2.65	0.029	1
1835	AP07620006	ABC transporter-like protein	8.96	5.5	64.24	4.76	99.17	2.22	0.00083	10
1901^p	AP05290001	Putative transposase	12.64	9.92	40.43	6.11	92.87	1.43	0.0093	6
Chaperones
1053	AP08040017	Chaperonin GroEL (HSP60 family)	5.73	4.89	58.72	5.61	173.26	1.5	0.0059	6
1451	AP04730007	Chaperone clpB 2	7.22	5.4	98.73	5.17	131.08	1.98	0.008	10
DNA damage/DNA reparing system
608^p	AP06420003	RNA-directed DNA polymerase	7.92	10.27	50.25	5.27	225.29	4.45	0.028	4
1278	AP05970008	DNA gyrase subunit A	5.69	5.16	61.11	4.97	148.42	2.47	0.0019	10
1209	AP02770002	Putative chromosome segregation ATPases	9.45	5.2	38.26	5.31	155.55	1.47	0.033	6
1789	AP06960003	Putative exonuclease SbcC	3.05	4.89	76.57	5.79	103.20	1.33	0.00039	6
1969	AP06870011	Type I restriction system endonuclease	4.07	6.4	119.09	5	88.20	1.7	0.038	19
3100	AP07900024	Restriction endonuclease	9.39	5.89	24.50	5.76	34.05	2.49	0.043	10
Translation machinery
1368	AP04960025	30S ribosomal protein S2	6.25	4.77	32.18	4.95	138.99	5.46	0.016	4
Channeling systems
1488	AP07910035	Outer membrane efflux protein	6.03	4.89	76.57	5.71	127.74	1.62	0.017	6
Hypothetical protein
520	AP07020011	Conserved hypothetical protein	1.48	5.46	249.83	4.8	235.55	1.44	0.026	6
1726	AP07780003	Conserved hypothetical protein	7.03	4.82	68.18	5.01	107.15	2.26	0.015	10
Proteins containing conserved motifs
537^p	AP06120011	TPR repeat-containing protein-O-linked GlcNAc transferase	5.01	4.88	72.71	5.42	234.45	1.49	0.032	19
1205	AP06120011	TPR repeat-containing protein-O-linked GlcNAc transferase	5.95	4.88	72.71	4.94	154.46	2.29	0.023	10
1212	AP06930009	TPR repeat-containing protein-O-linked GlcNAc transferase	6.66	5.94	71.42	5.38	155.55	1.5	0.0052	22
1358	AP06390003	WD-40 repeat protein-transcriptional regulator, XRE family	2.9	6.2	160.34	5.01	139.97	4.79	0.018	4
Others
840	AP06080002	Putative enzyme of poly-gamma-glutamate biosynthesis, capsule formation	8.29	5.59	72.57	5.3	199.90	3.31	0.019	4

^t Cluster types; (i) cluster 1, 2, 6, 16 and 19 are sustained tolerance proteins and (ii) cluster 4, 5, 8, 10 and 22 are undetermined-pattern proteins.

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ISSN: 1477-5956

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