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Table 2 Significantly up-regulated proteins identified in the soluble fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

     theoretical experimental    
spot# orf protein name %cov pI MW (kDa) pI MW (kDa) fold* t-test cluster t
Up regulated proteins (separated by pH range 3-10 in the first dimension)          
Two component systems          
1282 AP07350018 Hybrid sensor and regulator 4.51 5.16 157.04 9.24 125.42 5.21 0.047 22
1399 AP08000025 Two-component sensor histidine kinase 8.04 5.09 80.65 8.67 116.52 3.42 0.022 4
2377 AP07580004 Two-component sensor histidine kinase 7.38 5.26 133.55 7.69 51.45 1.51 0.012 16
2765 AP07580004 Two-component sensor histidine kinase 4.07 5.26 133.55 8.13 34.68 4.05 0.0035 4
Stress related proteins          
2693 AP04260002 NADPH-dependent FMN reductase 7.48 6.53 24.75 3.37 37.83 2.98 0.039 5
Translation machinery          
23 AP07220011 30S ribosomal protein S1 14.32 4.48 42.60 4.88 308.16 1.55 0.037 16
Hypothetical proteins          
410 AP07020011 Conserved hypothetical protein 2.74 5.46 249.83 3.61 228.97 2.86 0.026 1
Proteins containing conserved motif          
1736 AP06120011 TPR repeat-containing protein-O-linked GlcNAc transferase 5.79 4.88 72.71 3.76 91.01 1.54 0.05 16
Up regulated proteins (separated by pH range 4-7 in the first dimension)          
Two component systems          
599p AP03710004 Sensory box/GGDEF family protein 11.55 4.95 65.15 5.16 226.88 4.41 0.034 4
665 AP06710002 Multi-sensor signal transduction histidine kinase 5.45 5.25 116.20 5.03 220.07 1.75 0.047 6
727 AP07310007 Hybrid sensory kinase 2.82 5.34 100.91 5.13 209.99 1.87 0.039 19
735 AP06460007 Multi-sensor Hybrid Histidine Kinase 6.58 4.93 200.70 5.21 210.48 1.73 0.039 1
737 AP02950002 Putative response regulator receiver signal transduction histidine kinase 13.19 4.91 42.67 5.27 209.99 2.04 0.0073 10
788 AP08040014 Ethylene response sensor protein 6.1 4.92 120.25 5.32 203.21 5.64 0.0035 2
1072 AP04840005 Serine/threonine kinase with TPR repeat 7.03 8.37 82.39 5.92 172.45 1.57 0.026 6
1153 AP07580004 Two-component sensor histidine kinase 5.85 5.26 133.55 5.75 159.61 1.54 0.025 6
1343 AP07350018 Hybrid sensor and regulator 2.25 5.16 157.04 5.73 142.96 1.34 0.02 6
1357 AP07580004 Two-component sensor histidine kinase 2.37 5.26 133.55 4.9 139.65 3.73 0.0091 4
1377 AP07580004 Two-component sensor histidine kinase 5.51 5.26 133.55 5.21 137.37 3.29 0.022 5
1482 AP07670017 Two-component sensor histidine kinase 4.07 4.88 124.16 5.59 127.74 1.7 0.021 6
1787 AP07430015 Sensory box histidine kinase/response regulator 1.21 5.41 138.91 4.56 102.00 2.01 0.0012 19
1883p AP06710002 Multi-sensor signal transduction histidine kinase 5.74 5.25 116.20 5.94 94.40 1.48 0.021 6
2635 AP04840005 Serine/threonine kinase with TPR repeat 5.79 8.37 82.39 5.31 50.01 1.36 0.016 6
2940 AP08000025 Two-component sensor histidine kinase 4.23 5.09 80.65 5.2 38.73 1.42 0.035 6
Stress related proteins          
460 AP07250004 Glycosyl transferase, family 2 1.19 5.45 236.54 5.34 248.02 2.16 0.0088 8
512p AP07620006 ABC transporter-like protein 8.96 5.5 64.24 5.3 238.33 3.11 0.01 4
613 AP07180022 Putative aldehyde dehydrogenase 12.21 6.38 29.30 5.36 226.35 4.15 0.021 4
799 AP06960005 Fe-S oxidoreductase 4.58 5.83 60.23 5.21 202.26 4.87 0.017 4
1328 AP07510011 Ribitol type dehydrogenase protein 5.1 5.53 47.30 5.96 143.97 2.11 0.013 1
1753 AP06510003 Putative membrane carboxypeptidase 3.15 8.68 84.38 5.46 105.40 2.65 0.029 1
1835 AP07620006 ABC transporter-like protein 8.96 5.5 64.24 4.76 99.17 2.22 0.00083 10
1901p AP05290001 Putative transposase 12.64 9.92 40.43 6.11 92.87 1.43 0.0093 6
Chaperones          
1053 AP08040017 Chaperonin GroEL (HSP60 family) 5.73 4.89 58.72 5.61 173.26 1.5 0.0059 6
1451 AP04730007 Chaperone clpB 2 7.22 5.4 98.73 5.17 131.08 1.98 0.008 10
DNA damage/DNA reparing system          
608p AP06420003 RNA-directed DNA polymerase 7.92 10.27 50.25 5.27 225.29 4.45 0.028 4
1278 AP05970008 DNA gyrase subunit A 5.69 5.16 61.11 4.97 148.42 2.47 0.0019 10
1209 AP02770002 Putative chromosome segregation ATPases 9.45 5.2 38.26 5.31 155.55 1.47 0.033 6
1789 AP06960003 Putative exonuclease SbcC 3.05 4.89 76.57 5.79 103.20 1.33 0.00039 6
1969 AP06870011 Type I restriction system endonuclease 4.07 6.4 119.09 5 88.20 1.7 0.038 19
3100 AP07900024 Restriction endonuclease 9.39 5.89 24.50 5.76 34.05 2.49 0.043 10
Translation machinery          
1368 AP04960025 30S ribosomal protein S2 6.25 4.77 32.18 4.95 138.99 5.46 0.016 4
Channeling systems          
1488 AP07910035 Outer membrane efflux protein 6.03 4.89 76.57 5.71 127.74 1.62 0.017 6
Hypothetical protein          
520 AP07020011 Conserved hypothetical protein 1.48 5.46 249.83 4.8 235.55 1.44 0.026 6
1726 AP07780003 Conserved hypothetical protein 7.03 4.82 68.18 5.01 107.15 2.26 0.015 10
Proteins containing conserved motifs          
537p AP06120011 TPR repeat-containing protein-O-linked GlcNAc transferase 5.01 4.88 72.71 5.42 234.45 1.49 0.032 19
1205 AP06120011 TPR repeat-containing protein-O-linked GlcNAc transferase 5.95 4.88 72.71 4.94 154.46 2.29 0.023 10
1212 AP06930009 TPR repeat-containing protein-O-linked GlcNAc transferase 6.66 5.94 71.42 5.38 155.55 1.5 0.0052 22
1358 AP06390003 WD-40 repeat protein-transcriptional regulator, XRE family 2.9 6.2 160.34 5.01 139.97 4.79 0.018 4
Others          
840 AP06080002 Putative enzyme of poly-gamma-glutamate biosynthesis, capsule formation 8.29 5.59 72.57 5.3 199.90 3.31 0.019 4
  1. t Cluster types; (i) cluster 1, 2, 6, 16 and 19 are sustained tolerance proteins and (ii) cluster 4, 5, 8, 10 and 22 are undetermined-pattern proteins.
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