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Table 3 Significantly up-regulated proteins identified in the thylakoid membrane fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

    

theoretical

experimental

   

spot#

orf

protein name

%cov

pI

MW (kDa)

pI

MW (kDa)

fold*

t-test

cluster t

Up regulated proteins (separated by pH range 3-10 in the first dimension)

         

Two component systems

         

954

AP05090011

Multi-sensor signal transduction histidine kinase

5.69

5.23

135.61

6.19

161.10

2.1

0.048

13

1011

AP07350018

Hybrid sensor and regulator

3.42

5.16

157.04

4.92

150.14

1.37

0.047

8

1707

AP06710002

Multi-sensor signal transduction histidine kinase

7.52

5.25

116.20

5.02

81.51

1.78

0.0043

22

Chaperones

         

912

AP07830020

Molecular chaperone DnaK

7.38

4.78

68.30

3.52

168.46

1.91

0.0078

23

Stress related proteins

         

571

AP07830017

Polyphosphate kinase

4.16

5.47

82.79

4.4

219.16

1.6

0.042

23

1078

AP07620006

ABC transporter-like protein

5.62

5.5

64.24

3.98

142.91

1.43

0.0079

8

1366

AP05380002

DEAD/DEAH box helicase domain protein (membrane-helicase)

2.67

6.09

239.75

4.14

111.67

1.88

0.011

23

3925

AP05940003

Transcriptional regulator, LysR family

9.86

7.11

31.25

8.37

6.19

2.66

0.012

23

DNA repairing system

         

684

AP04930005

N-6 DNA methylase

8.7

5.24

59.46

3.32

206.66

2.23

0.039

23

1399p

AP05780004

Type I site-specific restriction-modification with related helicase system

10.55

5.97

127.07

7.33

107.30

1.59

0.022

8

Translation machinery

         

1634

AP07840003

30S ribosomal protein S18

28.17

10.58

8.36

4.95

87.88

1.62

0.037

8

Channeling system

         

1499

AP05960003

Preprotein translocase SecA subunit

6.67

5.14

105.74

5.8

98.14

1.88

0.023

22

Hypothetical proteins

         

564

AP07810017

Hypothetical protein

12.75

7.9

211.37

4.3

221.75

1.6

0.048

22

Proteins containing conserved motifs

         

1242

AP06700002

WD-40 repeat protein-peptidase C14, caspase catalytic subunit p20

4.37

5.26

183.10

4.13

122.68

1.68

0.019

23

1258

AP06390003

Pentapeptide repeat- transcriptional regulator, XRE family

10.82

6.2

160.34

5.43

121.53

1.64

0.0057

8

Others

          

1627

AP04100001

Glucose-inhibited division protein A

5.49

6.21

71.63

4.83

88.29

1.79

0.039

8

Up regulated proteins (separated by pH range 4-7 in the first dimension)

         

Two component systems

         

1513p

AP06990006

Phytochrome-like protein

7.12

5.5

104.01

5.78

101.22

1.72

0.0076

23

1954

AP07670017

Two-component sensor histidine kinase

4.35

4.88

124.16

5.07

63.09

1.85

0.051

ud

Stress related proteins

         

502

AP07700024

Putative transcriptional acitvator, Baf

15.77

6.59

28.74

5.73

232.21

2.37

0.04

21

701

AP06620002

Glycosyl transferase domain containing protein

7.29

5.92

134.25

5.03

205.94

1.69

0.025

23

781

AP08030034

Aldo/keto reductase

16.02

6.22

38.85

5.08

194.92

1.88

0.046

23

879

AP06740013

Ferredoxin-glutamate synthase

2.04

5.6

169.95

4.66

184.48

2.27

0.047

23

2082

AP04600003

S-adenosyl-L-homocysteine hydrolase

14.96

5.63

48.77

5.27

54.85

2.18

0.023

23

DNA damage/DNA reparing system

         

163

AP03680003

Putative RNA-directed DNA polymerase (Reverse transcriptase):HNH endonuclease

8.13

9.73

56.22

5.1

293.02

3.89

0.027

6

Proteins containing conserved motif

         

1852

AP05550005

TPR repeat-prenyl transferase

12.7

5.34

41.12

4.75

72.40

1.89

0.0071

23

Others

         

452

AP07910008

Adenylate cyclase

13.72

5.59

51.10

5.42

237.50

5.53

0.032

21

  1. ud means the protein expression pattern(s) cannot be clustered.
  2. t Cluster types; cluster 6, 8,13, 21, 22 and 23 are sustained tolerance proteins.