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Table 3 Significantly up-regulated proteins identified in the thylakoid membrane fraction after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

     theoretical experimental    
spot# orf protein name %cov pI MW (kDa) pI MW (kDa) fold* t-test cluster t
Up regulated proteins (separated by pH range 3-10 in the first dimension)          
Two component systems          
954 AP05090011 Multi-sensor signal transduction histidine kinase 5.69 5.23 135.61 6.19 161.10 2.1 0.048 13
1011 AP07350018 Hybrid sensor and regulator 3.42 5.16 157.04 4.92 150.14 1.37 0.047 8
1707 AP06710002 Multi-sensor signal transduction histidine kinase 7.52 5.25 116.20 5.02 81.51 1.78 0.0043 22
Chaperones          
912 AP07830020 Molecular chaperone DnaK 7.38 4.78 68.30 3.52 168.46 1.91 0.0078 23
Stress related proteins          
571 AP07830017 Polyphosphate kinase 4.16 5.47 82.79 4.4 219.16 1.6 0.042 23
1078 AP07620006 ABC transporter-like protein 5.62 5.5 64.24 3.98 142.91 1.43 0.0079 8
1366 AP05380002 DEAD/DEAH box helicase domain protein (membrane-helicase) 2.67 6.09 239.75 4.14 111.67 1.88 0.011 23
3925 AP05940003 Transcriptional regulator, LysR family 9.86 7.11 31.25 8.37 6.19 2.66 0.012 23
DNA repairing system          
684 AP04930005 N-6 DNA methylase 8.7 5.24 59.46 3.32 206.66 2.23 0.039 23
1399p AP05780004 Type I site-specific restriction-modification with related helicase system 10.55 5.97 127.07 7.33 107.30 1.59 0.022 8
Translation machinery          
1634 AP07840003 30S ribosomal protein S18 28.17 10.58 8.36 4.95 87.88 1.62 0.037 8
Channeling system          
1499 AP05960003 Preprotein translocase SecA subunit 6.67 5.14 105.74 5.8 98.14 1.88 0.023 22
Hypothetical proteins          
564 AP07810017 Hypothetical protein 12.75 7.9 211.37 4.3 221.75 1.6 0.048 22
Proteins containing conserved motifs          
1242 AP06700002 WD-40 repeat protein-peptidase C14, caspase catalytic subunit p20 4.37 5.26 183.10 4.13 122.68 1.68 0.019 23
1258 AP06390003 Pentapeptide repeat- transcriptional regulator, XRE family 10.82 6.2 160.34 5.43 121.53 1.64 0.0057 8
Others           
1627 AP04100001 Glucose-inhibited division protein A 5.49 6.21 71.63 4.83 88.29 1.79 0.039 8
Up regulated proteins (separated by pH range 4-7 in the first dimension)          
Two component systems          
1513p AP06990006 Phytochrome-like protein 7.12 5.5 104.01 5.78 101.22 1.72 0.0076 23
1954 AP07670017 Two-component sensor histidine kinase 4.35 4.88 124.16 5.07 63.09 1.85 0.051 ud
Stress related proteins          
502 AP07700024 Putative transcriptional acitvator, Baf 15.77 6.59 28.74 5.73 232.21 2.37 0.04 21
701 AP06620002 Glycosyl transferase domain containing protein 7.29 5.92 134.25 5.03 205.94 1.69 0.025 23
781 AP08030034 Aldo/keto reductase 16.02 6.22 38.85 5.08 194.92 1.88 0.046 23
879 AP06740013 Ferredoxin-glutamate synthase 2.04 5.6 169.95 4.66 184.48 2.27 0.047 23
2082 AP04600003 S-adenosyl-L-homocysteine hydrolase 14.96 5.63 48.77 5.27 54.85 2.18 0.023 23
DNA damage/DNA reparing system          
163 AP03680003 Putative RNA-directed DNA polymerase (Reverse transcriptase):HNH endonuclease 8.13 9.73 56.22 5.1 293.02 3.89 0.027 6
Proteins containing conserved motif          
1852 AP05550005 TPR repeat-prenyl transferase 12.7 5.34 41.12 4.75 72.40 1.89 0.0071 23
Others          
452 AP07910008 Adenylate cyclase 13.72 5.59 51.10 5.42 237.50 5.53 0.032 21
  1. ud means the protein expression pattern(s) cannot be clustered.
  2. t Cluster types; cluster 6, 8,13, 21, 22 and 23 are sustained tolerance proteins.