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Table 4 Significantly down-regulated proteins identified in the three subcellular fractions after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

      theoretical experimental    
spot# frac/pH range orf protein name %cov pI MW (kDa) pI MW (kDa) fold* t-test cluster t
Plasma membrane fraction           
2410 PM/4-7 AP07850026 Two-component hybrid sensor and regulator 6.97 5.13 143.09 4.81 52.64 -2 0.024 4
2459 PM/4-7 AP04660005 TPR repeat-hypothetical protein 7.89 6.2 33.86 4.74 51.95 -2.18 0.024 4
Soluble fraction           
1396 SOL/4-7 AP07820015 Putative WD-40 repeat protein 3.6 9.2 120.78 4.34 134.50 -1.55 0.01 18
2466 SOL/4-7 AP07540009 Two-component hybrid sensor and regulator 3.37 5.33 156.25 4.48 58.11 -1.8 0.019 8
Thylakoid membrane fraction           
Two component systems           
1193 TM/4-7 AP06440002 Twin-arginine translocation pathway signal 7.82 7.84 44.13 5.1 136.65 -2.12 0.051 ud
1781 TM/4-7 AP07540011 Two component response regulator 7.06 6.09 74.15 5.78 77.65 -1.54 0.028 14
2019 TM/4-7 AP06580007 Two-component hybrid sensor and regulator 6.94 4.36 127.39 5.64 58.10 -3.22 0.0092 17
Stress related proteins           
1393 TM/4-7 AP07870030 Molybdopterin oxidoreductase 11.14 8.29 82.17 5.07 112.15 -2.76 0.027 10
1539 TM/4-7 AP05060006 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase 9.86 5.18 87.57 6.84 100.21 -1.79 0.00015 10
DNA damage/DNA repairing system           
1512 TM/3-10 AP07840004 Ribonuclease II 10.27 4.93 76.91 4.54 97.91 -1.3 0.049 14
1316 TM/4-7 AP07740021 Chromosome segregation ATPases 6.79 4.97 83.23 5.26 123.95 -1.41 0.047 1
1457 TM/4-7 AP07790019 Putative site-specific DNA-methyltransferase (cytosine-specific) 11.11 8.39 55.18 5.07 106.41 -4.43 0.0054 12
2943 TM/3-10 AP07750017 UvrD/REP helicase 4.39 5.47 121.28 7.49 18.08 -2.01 0.003 10
Proteins containing conserved motif           
2219 TM/4-7 AP07230009 TPR repeat containing protein-hypothetical protein 16.61 4.72 31.94 6.44 47.21 -2.7 0.035 10
Others           
1301 TM/4-7 AP06400004 Slr0554 protein. 7.41 8.54 108.70 7.11 124.89 -6.45 0.02 17
2577 TM/3-10 AP06920007 Aldehyde-alcohol dehydrogenase 13.21 5.85 54.75 7.58 30.60 -1.83 0.046 10
3572 TM/4-7 AP07900036 Delta-9 desaturase 7.78 7.27 31.41 4.71 10.69 -2.43 0.032 9
  1. * Fold represents fold change value which is volume ratio of after the temperature upshift (180 min)/before the temperature upshift. Volume ratio refers to the ratio of the normalized volumes of a pair of spots (the same spot of before and after the temperature upshift), for example, a value of 2.0 represents a two-fold increase while -2.0 represents a two-fold decrease.
  2. p These protein spots are phosphorylated at Ser, Thr and Tyr residues, detected by western blot analysis before the temperature upshift (0 min) and after the temperature upshift (45, 90, 180 min).
  3. Frac/pH range and % cov represent fraction and pH range where proteins were separated in the first dimension, and %coverage, respectively.
  4. Some of the down regulated proteins cannot be clearly visualized on the spot map shown in Additional figures.
  5. ud means the protein expression pattern(s) cannot be clustered.
  6. t Cluster types; (i) in PM fraction, cluster 4 is undetermined-pattern proteins, (ii) in SOL fraction, cluster 18 is sustained tolerance proteins and cluster 8 is undetermined-pattern proteins and (iii) in TM fraction, cluster 12 is resistance proteins, cluster 1, 10, and 17 are adaptation proteins, and cluster 9 and 14 are sustained tolerance proteins.