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Table 4 Significantly down-regulated proteins identified in the three subcellular fractions after the immediate temperature upshift.

From: Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis

     

theoretical

experimental

   

spot#

frac/pH range

orf

protein name

%cov

pI

MW (kDa)

pI

MW (kDa)

fold*

t-test

cluster t

Plasma membrane fraction

          

2410

PM/4-7

AP07850026

Two-component hybrid sensor and regulator

6.97

5.13

143.09

4.81

52.64

-2

0.024

4

2459

PM/4-7

AP04660005

TPR repeat-hypothetical protein

7.89

6.2

33.86

4.74

51.95

-2.18

0.024

4

Soluble fraction

          

1396

SOL/4-7

AP07820015

Putative WD-40 repeat protein

3.6

9.2

120.78

4.34

134.50

-1.55

0.01

18

2466

SOL/4-7

AP07540009

Two-component hybrid sensor and regulator

3.37

5.33

156.25

4.48

58.11

-1.8

0.019

8

Thylakoid membrane fraction

          

Two component systems

          

1193

TM/4-7

AP06440002

Twin-arginine translocation pathway signal

7.82

7.84

44.13

5.1

136.65

-2.12

0.051

ud

1781

TM/4-7

AP07540011

Two component response regulator

7.06

6.09

74.15

5.78

77.65

-1.54

0.028

14

2019

TM/4-7

AP06580007

Two-component hybrid sensor and regulator

6.94

4.36

127.39

5.64

58.10

-3.22

0.0092

17

Stress related proteins

          

1393

TM/4-7

AP07870030

Molybdopterin oxidoreductase

11.14

8.29

82.17

5.07

112.15

-2.76

0.027

10

1539

TM/4-7

AP05060006

5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase

9.86

5.18

87.57

6.84

100.21

-1.79

0.00015

10

DNA damage/DNA repairing system

          

1512

TM/3-10

AP07840004

Ribonuclease II

10.27

4.93

76.91

4.54

97.91

-1.3

0.049

14

1316

TM/4-7

AP07740021

Chromosome segregation ATPases

6.79

4.97

83.23

5.26

123.95

-1.41

0.047

1

1457

TM/4-7

AP07790019

Putative site-specific DNA-methyltransferase (cytosine-specific)

11.11

8.39

55.18

5.07

106.41

-4.43

0.0054

12

2943

TM/3-10

AP07750017

UvrD/REP helicase

4.39

5.47

121.28

7.49

18.08

-2.01

0.003

10

Proteins containing conserved motif

          

2219

TM/4-7

AP07230009

TPR repeat containing protein-hypothetical protein

16.61

4.72

31.94

6.44

47.21

-2.7

0.035

10

Others

          

1301

TM/4-7

AP06400004

Slr0554 protein.

7.41

8.54

108.70

7.11

124.89

-6.45

0.02

17

2577

TM/3-10

AP06920007

Aldehyde-alcohol dehydrogenase

13.21

5.85

54.75

7.58

30.60

-1.83

0.046

10

3572

TM/4-7

AP07900036

Delta-9 desaturase

7.78

7.27

31.41

4.71

10.69

-2.43

0.032

9

  1. * Fold represents fold change value which is volume ratio of after the temperature upshift (180 min)/before the temperature upshift. Volume ratio refers to the ratio of the normalized volumes of a pair of spots (the same spot of before and after the temperature upshift), for example, a value of 2.0 represents a two-fold increase while -2.0 represents a two-fold decrease.
  2. p These protein spots are phosphorylated at Ser, Thr and Tyr residues, detected by western blot analysis before the temperature upshift (0 min) and after the temperature upshift (45, 90, 180 min).
  3. Frac/pH range and % cov represent fraction and pH range where proteins were separated in the first dimension, and %coverage, respectively.
  4. Some of the down regulated proteins cannot be clearly visualized on the spot map shown in Additional figures.
  5. ud means the protein expression pattern(s) cannot be clustered.
  6. t Cluster types; (i) in PM fraction, cluster 4 is undetermined-pattern proteins, (ii) in SOL fraction, cluster 18 is sustained tolerance proteins and cluster 8 is undetermined-pattern proteins and (iii) in TM fraction, cluster 12 is resistance proteins, cluster 1, 10, and 17 are adaptation proteins, and cluster 9 and 14 are sustained tolerance proteins.
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